BRD4 Antibody

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• Catalog number
  6644-100
• Price:

  354.86 USD

  Ask for price
• Size
  100 μg

# #

• Antibody Target
  BRD4
• Target Alternative Name
  BRD4, Protein HUNK1, HUNK1
• Antibody Type
  Polyclonal
• Species Reactivities
  Human
• Immunogen Sequence
  Recombinant protein BRD4 (49-170 aa)(BV-M68)
• Accession
  O60885
• Gene Id
  23476
• Appearance
  Colorless liquid
• Form
  Liquid
• Concentration
  0.5 mg/ml
• Formulation
  100 µg (0.5 mg/ml) of antibody in PBS pH 7.2 containing 0.01 % BSA, 0.01 % thimerosal, and 50 % glycerol.
• Purification
  Affinity Purified Antibody
• Application
  Western blot
• Positive Control
  Western blot: Recombinant protein
• Application And Usages
  Western blot: ~1:200
• Description
  The acetylation of histone lysine residues plays a crucial role in the epigenetic regulation of gene transcription. A bromodomain is a protein domain that recognizes acetylated lysine residues such as those on the N-terminal tails of histones. This recognition is often a prerequisite for protein-histone association and chromatin remodeling. These domains function in the linking of protein complexes to acetylated nucleosomes, thereby controlling chromatin structure and gene expression. Thus, bromodomains serve as “readers” of histone acetylation marks regulating the transcription of target promoters. The BET family of proteins, defined by tandem Bromodomains and an Extra Terminal domain, include BRD2, BRD3, BRD4, and BRDT. The BET proteins play a key role in many cellular processes, including inflammatory gene expression, mitosis, and viral/host interactions. The isolated individual or tandem bromodomains of BRD2 and BRD4 have been shown to bind acetylated histone tails, serving to couple histone acetylation marks to the transcriptional regulation of target promoters. Small molecule inhibitors of these interactions hold promise as useful therapeutics for human disease.
• Storage Condition
  -20 °C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans.
• Handling
  The antibody solution should be gently mixed before use.
• Western Blot Verified
  Verified
• Immunocytochemistry Verified
  Not Verified
• Immunofluorescence Verified
  Not Verified
• Immunoprecipitation Verified
  Not Verified
• ELISA Verified
  Not Verified
• ChIP Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

#

• Gene target
  BRD4  
• Gene symbol
  BRD4
• Short name
  BRD4 Antibody
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  BRD4 (antibody to-)
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:13575
• Gene
  BRD4
• Long gene name
  bromodomain containing 4
• Synonyms gene name
  • bromodomain-containing 4
• Synonyms
  • HUNKI
  • MCAP
  • CAP
  • HUNK1
• Synonyms name
  • chromosome-associated protein
  • mitotic chromosome-associated protein
• GenBank acession
  • Y12059
• Locus
  19p13.12
• Discovery year
  2000-09-26
• Entrez gene record
  23476
• Pubmed identfication
  • 10938129
• RefSeq identity
  • NM_058243
• Classification
  • Bromodomain containing
  • Bromodomain and extra-terminal domain family
• VEGA ID
  OTTHUMG00000183252

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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