anti-Amphiphysin Antibody

• Catalog number
  45-006
• Price
  Please ask
• Size
  0.1 mg

• Gene names
  AMPH, amphiphysin (Stiff-Man syndrome with breast cancer 128 kDa autoantigen), HGNC:471, AMPH1, amphiphysin, amphiphysin (Stiff-Mann syndrome with breast cancer 128kD autoantigen)
• Antibodie s label
  unlabelled serum
• Purity
  Amphiphysin antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Ab raised in
  Goat
• Type
  Polyclonal
• Spicific to
  This antibody is expected to recognize both reported isoforms (NP_001626.1 and NP_647477.1).
• Antigen
  Amphiphysin antibody was made against a 12 amino acid synthetic peptide near the internal region of Amphiphysin.
• Immuno application
  ELISA, Western Blot, immuno-histo-chemistry
• Antibody advice
  ELISA: Antibody detection limit dilution 1:16000. Western Blot: Approximately 125 kDa band observed in mouse brain lysates (calculated MW of 76.3 kDa according to NP_001626.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Wigge et al, Mol Biol Cell. 1997 Oct;8 (10) :2003-15.; PMID: 9348539). Recommended concentration: 0.1-0.5 ug/mL. A minor band of unknown identity was also consistently observed at 45 kDa . This band was successfully blocked by incubation with the immunizing peptide. Immunohistochemistry: In paraffin embedded human cerebellum shows nuclear and fibrous staning. Recommended concentration, 2-4 ug/mL.
• Suspention pH azide
  AmpH ipH ysin antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
• Keep at
  Aliquot and store at -20 grades C. Minimize freezing and thawing.
• Works with
  Human, Mouse
• Omim nr
  4502081
• Ncbi nr
  NP_001626.1, NP_647477.1
• Vial with antibody
  in solution
• Quantity per ml
  500 ug/mL
• NCBI GENE nr
  273
• GENE symbol
  AMPH
• Antigene name
  amphiphysin
• Source
  Homo sapiens
• Protein nr
  P49418
• Picture
• Pdf tech file
• More antibodies
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Properties
  If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  Amphiphysin  
• Gene symbol
  AMPH, BIN1
• Short name
  anti-Amphiphysin Antibody
• Technique
  Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
• Alternative name
  Antibody toAmphiphysin (antibody to-)
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:471
• Gene
  AMPH
• Long gene name
  amphiphysin
• Synonyms gene name
  • amphiphysin (Stiff-Mann syndrome with breast cancer 128kD autoantigen)
  • amphiphysin (Stiff-Man syndrome with breast cancer 128kDa autoantigen)
• Locus
  7p14.1
• Discovery year
  1994-12-12
• Entrez gene record
  273
• Pubmed identfication
  • 8245793
• RefSeq identity
  • NM_001635
• Classification
  • N-BAR domain containing
• VEGA ID
  OTTHUMG00000023725

Gene info

• Identity
  HGNC:1052
• Gene
  BIN1
• Long gene name
  bridging integrator 1
• Synonyms gene
  • AMPHL
• Synonyms
  • SH3P9
  • AMPH2
• Synonyms name
  • amphiphysin II
• GenBank acession
  • U68485
• Locus
  2q14.3
• Discovery year
  2000-05-19
• Entrez gene record
  274
• Pubmed identfication
  • 8725406
  • 8782822
  • 17676042
• RefSeq identity
  • NM_139343
• Classification
  • N-BAR domain containing
• VEGA ID
  OTTHUMG00000131465
• Locus Specific Databases
  • LRG_873

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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