MBD4 Antibody
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Catalog number3056-30T
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PricePlease ask
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Size30 µg
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Antibody TargetMBD4
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Target Alternative NameMBD4, Methyl-CpG-binding domain protein 4, Methyl-CpG-binding endonuclease 1, Methyl-CpG-binding protein, MBD4, Mismatch-specific DNA N-glycosylase, MED1
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Antibody TypePolyclonal
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Species ReactivitiesHuman, mouse, rat
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Immunogen SequenceSynthetic peptide surrounding amino acid 474 of mouse MBD4
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AccessionO95243
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Gene Id8930
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AppearanceColorless liquid
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FormLiquid
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Concentration0.5 mg/ml
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Formulation30 µg (0.5 mg/ml) affinity purified rabbit anti-MBD4 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.01% thimerosal.
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PurificationAffinity purified
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ApplicationWestern blot
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Positive ControlJurkat cell lysates
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Application And UsagesThe antibody can be used in Western Blot analysis (0.5-4 µg/ml). However, the optimal concentrations should be determined individually.
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DescriptionMBD4 (methyl-CpG-binding domain protein 4) belongs to the MBD family of nuclear proteins that bind to methylated CpG sites and represses gene transcription. Human proteins MECP2, MBD1, MBD2, and MBD4, except MBD3 bind specifically to methylated DNA. MBD4 exhibits thymine glycosylase activity and is known to play an important function in DNA mismatch repair and apoptosis.
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Storage Condition-20 °C
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Shipping Conditiongel pack
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Shelf Life12 months
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UsageFor Research Use Only! Not to be used in humans.
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HandlingThe antibody solution should be gently mixed before use.
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Western Blot VerifiedVerified
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Immunocytochemistry VerifiedNot Verified
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Immunofluorescence VerifiedNot Verified
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Immunoprecipitation VerifiedNot Verified
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FACS VerifiedNot Verified
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ELISA VerifiedNot Verified
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ChIP VerifiedNot Verified
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Flow Cytometry VerifiedNot Verified
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PropertiesIf you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolMBD4
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Short nameMBD4 Antibody
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeRabbit IgG
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Alternative namemethyl-CpG binding domain protein 4 (antibody to-)
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Alternative techniqueantibodies
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Alternative to gene targetmethyl-CpG binding domain protein 4, MED1, MBD4 and IDBG-55837 and ENSG00000129071 and 8930, pyrimidine-specific mismatch base pair DNA N-glycosylase activity, nuclei, Mbd4 and IDBG-179645 and ENSMUSG00000030322 and 17193, BT.49400 and IDBG-646370 and ENSBTAG00000021645 and 511463
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Gene info
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Identity
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Gene
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Long gene namemethyl-CpG binding domain 4, DNA glycosylase
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Synonyms gene name
- methyl-CpG binding domain protein 4
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Synonyms
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GenBank acession
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Locus
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Discovery year1999-01-11
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- DNA glycosylases
- Methyl-CpG binding domain containing
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data