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Long name
Aurora A (Thr288) Polyclonal Antibody, ALEXA FLUOR 594 Conjugated
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Also known as
Anti-Aurora A Thr288 PAb ALEXA FLUOR 594
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Category
Conjugated Primary Antibodies
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Conjugated with
ALEXA FLUOR® 594
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Host Organism
Rabbit (Oryctolagus cuniculus)
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Target Antigen
Aurora A Thr288
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Specificity
This is a highly specific antibody against Aurora A Thr288.
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Modification
Phosphorylation
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Modification Site
Thr288
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Clonality
Polyclonal
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Clone
Polyclonal antibody
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Concentration
1ug per 1ul
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Immunogen range
260-300/403
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Subcellular location
Cytoplasm, Nucleus
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Source
KLH conjugated synthetic phosphopeptide derived from human Aurora A around the phosphorylation site of Thr288
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Gene ID Number
6790
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Swiss Prot
O14965
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Tested applications
IF(IHC-P)
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Recommended dilutions
IF(IHC-P)(1:50-200)
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Crossreactivity
Human, Rat
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Cross reactive species details
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
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Background of the antigen
Mitotic serine/threonine kinases that contributes to the regulation of cell cycle progression. Associates with the centrosome and the spindle microtubules during mitosis and plays a critical role in various mitotic events including the establishment of mitotic spindle, centrosome duplication, centrosome separation as well as maturation, chromosomal alignment, spindle assembly checkpoint, and cytokinesis. Required for initial activation of CDK1 at centrosomes. Phosphorylates numerous target proteins, including ARHGEF2, BORA, BRCA1, CDC25B, DLGP5, HDAC6, KIF2A, LATS2, NDEL1, PARD3, PPP1R2, PLK1, RASSF1, TACC3, p53/TP53 and TPX2. Regulates KIF2A tubulin depolymerase activity. Required for normal axon formation. Plays a role in microtubule remodeling during neurite extension. Important for microtubule formation and/or stabilization. Also acts as a key regulatory component of the p53/TP53 pathway, and particularly the checkpoint-response pathways critical for oncogenic transformation of cells, by phosphorylating and stabilizing p53/TP53. Phosphorylates its own inhibitors, the protein phosphatase type 1 (PP1) isoforms, to inhibit their activity. Necessary for proper cilia disassembly prior to mitosis.
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Purification
Purified by Protein A.
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Storage conditions
Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.
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Excitation emission
590nm/617nm
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Synonyms
AIK; ARK1; AURA; BTAK; STK6; STK7; STK15; AURORA2; PPP1R47; Aurora kinase A; Aurora 2; Aurora/IPL1-related kinase 1; ARK-1; Aurora-related kinase 1; hARK1; Breast tumor-amplified kinase; Serine/threonine-protein kinase 15; Serine/threonine-protein kinase 6; Serine/threonine-protein kinase aurora-A; AURKA; AIRK1; AYK1; IAK1
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Properties
For facs or microscopy Alexa 1 conjugate. If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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Conjugation
Alexa Fluor, ALEXA FLUOR® 594
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Conjugated
Alexa conjugate 1
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French translation
anticorps
