ASH2 polyclonal antibody
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Catalog number6836-50
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PricePlease ask
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Size50 μl
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Antibody TargetASH2
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Target Alternative NameASH2L, ASH2L1, ASH2L2, Bre2
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Antibody TypePolyclonal
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Species ReactivitiesMouse.
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Immunogen SequencePolyclonal antibody raised in rabbit against mouse Ash2 (absent, small, or homeotic 2), using 3 different KLH-conjugated synthetic peptides, 2 containing an amino acid sequence from the central and 1 containing an amino acid sequence from the C-terminal part of the protein.
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AccessionQ9UBL3
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Gene Id9070
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AppearanceColorless liquid
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FormLiquid
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FormulationIn PBS with 0.05% (W/V) sodium azide.
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PurificationWhole Anti-Sera
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ApplicationWestern blot, ELISA.
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Positive ControlWestern blot: mouse fibroblasts and neural stem cells, ELISA: Peptides, IF: NIH3T3 cells.
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Application And UsagesELISA: 1:100 – 1:500, Western Blotting: 1:500 – 1:1000, IF: 1:200.
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DescriptionAsh2 is a component of the Set1/Ash2 histone methyltransferase (HMT) complex. This complex specifically methylates K4 of histone H3, thereby activating transcription. Methylation of K4 is blocked by premethylation of the neighboring K9, a repressor of transcription. This indicates that the Set1/Ash2 HMT complex mediates the crosstalk between K9 methylation and K4 methylation. Ash2 plays a role in hematopoiesis and may be associated with some kinds of leukemia.
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Storage Condition-20 °C
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Shipping Conditiongel pack
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Shelf Life12 months
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UsageFor Research Use Only! Not to be used in humans.
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HandlingThe antibody solution should be gently mixed before use.
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Western Blot VerifiedNot Verified
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Immunocytochemistry VerifiedNot Verified
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Immunofluorescence VerifiedNot Verified
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Immunoprecipitation VerifiedNot Verified
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ELISA VerifiedVerified
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ChIP VerifiedVerified
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Dot Blot VerifiedNot Verified
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Flow Cytometry VerifiedNot Verified
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PropertiesIf you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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GroupPolyclonals and antibodies
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AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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French translationanticorps
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Gene target
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Gene symbolASCL2, ASH2L
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Short nameASH2 polyclonal antibody
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TechniquePolyclonal, Antibody, antibodies against human proteins, antibodies for, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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IsotypeRabbit IgG
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Alternative nameASH2 polyclonal (antibody to-)
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Alternative techniquepolyclonals, antibodies
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Gene info
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Identity
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Gene
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Long gene nameachaete-scute family bHLH transcription factor 2
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Synonyms gene name
- achaete-scute complex (Drosophila) homolog-like 2
- achaete-scute complex-like 2 (Drosophila)
- achaete-scute complex homolog 2 (Drosophila)
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Synonyms
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Locus
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Discovery year1996-04-12
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Basic helix-loop-helix proteins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameASH2 like, histone lysine methyltransferase complex subunit
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Synonyms gene
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Synonyms gene name
- ash2 (absent, small, or homeotic)-like (Drosophila)
- ASH2 like histone lysine methyltransferase complex subunit
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-12-03
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- WRAD complex
- PHD finger proteins
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data