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Type
Conjugated Primary Antibody
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Conjugated with
ALEXA FLUOR 594
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Host organism
Mouse (Mus musculus)
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Target Protein Peptide
Metronidazole
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Specificity
Highly specific antibody against Metronidazole
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Modification
Not modified
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Clonality
Monoclonal Antibodies
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Clone
1C2
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Concentration
1ug per 1ul
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Antigen Source
KLH conjugated with Metronidazole
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Gene ID
N/A
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Applications
IF(IHC-P)
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Applications with corresponding dilutions
IF(IHC-P)(1:50-200)
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Cross Reactive species
Others
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Cross Reactive Species details
Metronidazole
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Background information
Metronidazole is a nitroimidazole antibiotic medication used particularly for anaerobic bacteria and protozoa. Metronidazole is an antibiotic, amebicide, and antiprotozoal.Metronidazole, taken up by diffusion, is selectively absorbed by anaerobic bacteria and sensitive protozoa. Once taken up by anaerobes, it is non-enzymatically reduced by reacting with reduced ferredoxin, which is generated by pyruvate oxido-reductase. Many of the reduced nitroso intermediates will form sulfinamides and thioether linkages with cysteine-bearing enzymes, thereby deactivating these critical enzymes. As many as 150 separate enzymes are affected.In addition or alternatively, the metronidazole metabolites are taken up into bacterial DNA, and form unstable molecules. This function only occurs when metronidazole is partially reduced, and because this reduction usually happens only in anaerobic cells, it has relatively little effect upon human cells or aerobic bacteria.
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Purification method
Purified by Protein G.
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Storage
Aqueous buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
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Excitation emission
590nm/617nm
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Synonyms
2-Methyl-5-nitroimidazole-1-ethanol
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Alternative name 1
Metronidazole(1C2) Monoclonal Antibody, ALEXA FLUOR® 594 Conjugated
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Alternative name 2
Metronidazole(1C2) Monoclonal anti-, ALEXA FLUOR 594 Conjugated
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Advisory
Cycles of freezing and thawing should be avoided as such cycles may denature the peptide chains of the antibody and reduce its affinity, specificity and reactivity. For antibodies in a liquid form or reconstituted lyophilized antibodies - small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.
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Properties
For facs or microscopy Alexa 1 conjugate.
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Conjugation
Alexa Fluor, ALEXA FLUOR® 594
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Conjugated
Alexa conjugate 1
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.