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Available ordering format
Lyophilized
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Immunogen
partly aggregated, recombinant peptide corresponding to the human Abeta (1-40/42). Amino acid sequence: D-A-E-F-R-H-D-S-G-Y-E-V-H-H-Q-K-L-V-F-F-A-E-D-V-G-S-N-K-G-A-I-I-G-L-M-V-G-G-V-V. The epitope is 3-8.
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Raised in
Mouse
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Clonality
Monoclonal
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Clone
IgM
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Purification
Affinity purified
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How to reconstitute
For reconstitution add 100 µl of sterile water.
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Storage condition
store lyophilized/reconstituted at 4°C. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
ELISA (ELISA), immunohistochemistry (IHC)
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Connected products
AS13 2716 | mAB-M | human Abeta protein (3-10) region, oligomer-specific, mouse monoclonal antibodyAS13 2715 | mAB-O | human Abeta protein (3-10) region, oligomer specific, mouse monoclonal antibodyAgrisera matching secondary antibody: Goat anti-mouse IgM (µ chain), HRP conjugated, min. cross-reactivity to human IgG/serum, AS10 969Secondary antibodies
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Recommended dilutions for use
coating antibody at 2 µg/ml (ELISA), 1: 500 (IHC)
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Molecular weight expected аpparent
4.5 kDa
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Verified reactivity
human Abeta oligomers only
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Possible reactivity
rat
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No reactivity
no confirmed exceptions from predicted reactivity known in the moment
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Supplementary information
OMAB antibody is a versatile tool within research of Alzheimer-™s disease. A sandwhich ELISA illustrates its potential regarding its high selectivity towards Aβ oligomers.
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References
Zhao et al. (2016). Antiamyloidogenic Activity of Aβ42-Binding Peptoid in Modulating Amyloid Oligomerization. Small. 2016 Oct 7. doi: 10.1002/smll.201602857.Richman et al. (2013). In Vitro and Mechanistic Studies of an Anti-Amyloidogenic Self-Assembled Cyclic D,L-#-Peptide Architecture. J. Americal Chemical Societ, Jan 19.Lindhagen-Persson et al. (2010). Amyloid-β Oligomer Specificity Mediated by the IgM Isotype - Implications for a Specific Protective Mechanism Exerted by Endogenous Auto-Antibodies. PLoS ONE.
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Scientific context
Soluble oligomeric assemblies of the Amyloid-β peptide are today anticipated to be the direct cause regarding the Alzheimer pathology. As a consequence, oligomeric Aβ-assemblies constitute a very interesting therapeutic target. Identification of Aβ-oligomers is however, technically challenging due to there labile nature and low abundance. Abeta oligomer-specific OMAB antibody is based on the IgM isotype and represents a new concept of Aβ-oligomer binders using a combination of high avidity and very low monovalent affinity. This combination creates a selectivity of the antibody towards the oligomeric fraction and minimizes reactivity towards monomeric species.
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Notes
OMAB antibody has been purified by by ion-exchange chromatography and is supplied in PBS without any additives as carrier proteins or sodium azide. Binding of OMAB antibody and Abeta oligomers at RT takes about 15 min.Fibrils are inaccessible for OMAB antibodies therefore if a discrimination between fibrils and oligomers is to be achieved, dot blot can be used. Start with antigen concentration of 500 ng/dot followed by 2X dilution steps. Blocking: non-fat milk and washes with 0.3 % Tween 20 in TBS pH 7.4.
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Protein number
Refer to NCBI
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TAIR number
Refer to NCBI
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Properties
If you buy Antibodies supplied by agisera they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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French translation
anticorps
