AMPK alpha 2 Antibody
AMPK alpha 2 Antibody is available 0 time from Bioss primary unconjugated antibodies labs
- Long nameAMPK alpha 2 Primary Polyclonal Antibody
- Also known asAMPK alpha 2 PAb
- CategoryPrimary Antibodies
- Target AntigenAMPK alpha 2
- SpecificityThis is a highly specific antibody against AMPK alpha 2.
- Modification site(s)Unmodified antibody
- ClonalityPolyclonal antibody
- Clone numberPolyclonal antibody
- Concentration1ug per 1ul
- Immunogen range375-425/552
- Subcellular locationCytoplasm, Nucleus
- SourceThis antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human AMPK alpha 2
- Gene ID number5563
- Swiss ProtP54646
- Tested ApplicationsWB, IHC-P, IF(IHC-P)
- Recommended dilutionsWB(1:100-1000), IHC-P(1:100-500), IF(IHC-P)(1:50-200)
- Cross reactivityHuman, Mouse, Rat
- Cross reactive species detailsDue to limited amount of testing and knowledge, not every possible cross-reactivity is known.
- Background of the target antigenCatalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Regulates lipid synthesis by phosphorylating and inactivating lipid metabolic enzymes such as ACACA, ACACB, GYS1, HMGCR and LIPE; regulates fatty acid and cholesterol synthesis by phosphorylating acetyl-CoA carboxylase (ACACA and ACACB) and hormone-sensitive lipase (LIPE) enzymes, respectively. Regulates insulin-signaling and glycolysis by phosphorylating IRS1, PFKFB2 and PFKFB3. AMPK stimulates glucose uptake in muscle by increasing the translocation of the glucose transporter SLC2A4/GLUT4 to the plasma membrane, possibly by mediating phosphorylation of TBC1D4/AS160. Regulates transcription and chromatin structure by phosphorylating transcription regulators involved in energy metabolism such as CRTC2/TORC2, FOXO3, histone H2B, HDAC5, MEF2C, MLXIPL/ChREBP, EP300, HNF4A, p53/TP53, SREBF1, SREBF2 and PPARGC1A. Acts as a key regulator of glucose homeostasis in liver by phosphorylating CRTC2/TORC2, leading to CRTC2/TORC2 sequestration in the cytoplasm. In response to stress, phosphorylates 'Ser-36' of histone H2B (H2BS36ph), leading to promote transcription. Acts as a key regulator of cell growth and proliferation by phosphorylating TSC2, RPTOR and ATG1/ULK1: in response to nutrient limitation, negatively regulates the mTORC1 complex by phosphorylating RPTOR component of the mTORC1 complex and by phosphorylating and activating TSC2.
- Purification methodThis antibody was purified via Protein A.
- Storage conditionsKeep the antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for up to 1 year.
- Synonym namesAMPK; AMPK2; PRKAA; AMPKa2; 5'-AMP-activated protein kinase catalytic subunit alpha-2; AMPK subunit alpha-2; Acetyl-CoA carboxylase kinase; ACACA kinase; Hydroxymethylglutaryl-CoA reductase kinase; HMGCR kinase; PRKAA2
- DescriptionThe AMPK alpha 2 Antibody is a α- or alpha protein sometimes glycoprotein present in blood.
- PropertiesIf you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
- French translationanticorps
- Gene target
- Short nameAMPK alpha 2 Antibody
- Alternative nameAMPK a 2 (Antibody to)
- Alternative techniqueantibodies