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Target antigen
ADAM17
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Clonality
Polyclonal antibody
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Clone
Polyclonal antibody
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Raised in
rabbit
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Type of the antibody
IgG polyclonal antibody
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Product form
freeze-dried
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Reacts with species
human, rat Predicted to work with: mouse
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Analyses
WB
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Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human ADAM17(806-824aa AASFKLQRQNRVDSKETEC), different from the related rat and mouse sequences by one amino acid.
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Product configuration
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
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Purification
Immunogen affinity purified.
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Solubilization
The powdered antibody should be dissolved in 0.2 ml of distilled water to achieve final concentration of 500ug/ml
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Storage condtions
Keep the ADAM17 Antibodyat minus twenty degrees Celsius for 1 year. The ready-to-use solutions can be stored at four degrees Celsius for a month. Our specialsits recommend to freeze the aliquotes at minus twenty degrees Celsius for long-term application. Multiple procedures of freezing and thawing influence the specifity and reactivity of the antibody in a negative way.
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Tips
The ADAM17 Antibody did not cross-reacted with other proteins during the test procedures. This antobdy is intended to be used for research analyses and it is not applicale for in vitro diagnostics.
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Background
ADAM17(ADAM metallopeptidase domain 17), also called TACE (tumor necrosis factor-alpha-converting enzyme), is a 70-kDa enzyme that belongs to the ADAM protein family of disintegrins and metalloproteases. Expression studies showed that the encoded protein cleaves precursor tumor necrosis factor-alpha to its mature form. Northern blot analysis revealed that the gene was expressed as a 5-kb mRNA in all tissues examined. ADAM17 is understood to be involved in the processing of tumor necrosis factor alpha(TNF-alpha) at the surface of the cell, and from within theintracellular membranes of the trans-Golgi network. This process, which is also known as "shedding", involves the cleavage and release of a soluble ectodomain from membrane-bound pro-proteins(such as pro-TNF-alpha), and is of known physiological importance. ADAM17 was the first "sheddase" to be identified, and is also understood to play a role in the release of a diverse variety of membrane-anchored cytokines, cell adhesion molecules,receptors, ligands, and enzymes.
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Related articles
1. Black, R. A., Rauch, C. T., Kozlosky, C. J., Peschon, J. J., Slack, J. L., Wolfson, M. F., Castner, B. J., Stocking, K. L., Reddy, P., Srinivasan, S., Nelson, N., Boiani, N., Schooley, K. A., Gerhart, M., Davis, R., Fitzner, J. N., Johnson, R. S., Paxton, R. J., March, C. J., Cerretti, D. P. A metalloproteinase disintegrin that releases tumour-necrosis factor-alpha from cells. Nature 385: 729-733, 1997.
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Gene Name
ADAM17
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Protein Name
Disintegrin and metalloproteinase domain-containing protein 17(ADAM 17)
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Gene Full Name
ADAM metallopeptidase domain 17
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Synonyms
A disintegrin and metalloproteinase domain 17(tumor necrosis factor, alpha, converting enzyme) antibody|A disintegrin and metalloproteinase domain 17 antibody|ADA17_HUMAN antibody|ADAM 17 antibody|ADAM metallopeptidase domain 17 antibody|ADAM17 antibody|ADAM17 protein antibody|CD 156b antibody|CD156b antibody|CD156b antigen antibody|CSVP antibody|Disintegrin and metalloproteinase domain-containing protein 17 antibody|MGC71942 antibody|Snake venom like protease antibody|Snake venom-like protease antibody|TACE antibody|TNF alpha convertase antibody|TNF alpha converting enzyme antibody|TNF-alpha convertase antibody|TNF-alpha-converting enzyme antibody|Tumor Necrosis Factor Alpha Converting Enzyme antibody
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Uniprot ID
P78536
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Properties
If you buy Antibodies supplied by boster they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
