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Synonyms
TG2, tTG, tissue transglutaminase, transglutaminase C, TGC, protein-glutamine-gamma-glutamyltransferase, TGM2
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Alternative_names
TG2, tTG, tissue transglutaminase, transglutaminase C, TGC, protein-glutamine-gamma-glutamyltransferase, TGM2
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Description
His tagged Human Transglutaminase 2 from E.Coli, AOF, ≥90% pure, biologically active.
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Recombinant
Yes
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Source
E. coli.
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Purity by SDS PAGE
≥90%
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Assay
SDS-PAGE
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Activity Specifications test method
BioVision’s Active His-tagged TG2 has been tested for its activity. It has a specific activity of >400 mU/mg. 1 U of TG2 activity is the amount of enzyme that will catalyze the formation of 1 umol of hydroxamate per min as measured using Transglutaminase Activity Assay Kit (Colorimetric) (BioVision Cat # K571-100).
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Biological activity
>400 mU/mg
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Results
>400 mU/mg
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Unit Definition
1 U of TG2 activity is the amount of enzyme that will catalyze the formation of 1 umol of hydroxamate per min as measured using Transglutaminase Activity Assay Kit (Colorimetric) (BioVision Cat # K571-100).
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Molecular Weight
79.5 kDa (1-687 aa + N-terminal polyhistidine tag).
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Storage Temp
-20°C
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Shipping
gel pack
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Shelf Life
12 months
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Appearance
Lyophilized powder
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Physical form description
Lyophilized from 5 mg/ml solution in a proprietary buffer.
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Reconstitution Instructions
Reconstitute to 0.1-0.5 mU/µl in 20 mM Tris, 120 mM NaCl, 10 mM DTT, pH 7.5 containing 20% glycerol. Aliquot and store at –80°C. Avoid repeated freezing and thawing cycles.
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Background Information
Tissue transglutaminase, a 78-kDa calcium dependent enzyme (EC 2.3.2.13), is found both in the intracellular and the extracellular spaces of various types of tissues. TG2 crosslinks proteins between the ε-amino group of a lysine residue and the γ-carboxamide group of glutamine residue, creating an inter- or intramolecular bond that is highly resistant to proteolysis (protein degradation). TG2 also possesses deamidation, GTP-binding/hydrolyzing, and isopeptidase activities. Intracellular TG2 is thought to play an important role in apoptosis, while extracellular TG2 has been linked to cell adhesion, ECM stabilization, wound healing, receptor signaling, cellular proliferation, and cellular motility.
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Amino acid sequence
1-687 aa of human transglutaminase 2 + N-terminal polyhistidine tag
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Conjugation
histidine
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional source
Recombinants or rec. proteins
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Group
recombinants
