T-COR 8 Real-time PCR Thermocycler

Group

PCR, polymerase chain reaction
About

TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield. Real-time PCR amplification mixes are available for quantification with specific selected primers and with optimized buffers.
Properties

Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.
Description

Real time PCR kits are used with DNA extraction kits supplied in 50 or 100 tests with polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR quantitatively (Quantitative real-time PCR), semi-quantitatively. RNA is often converted to cDNA. Often used as diagnostic tool. Ct values will have to be set for your probes.

Gene target

T-COR Thermocycler
Gene symbol

TBXT, PLS3, NCOR1, ABAT, DBET, TBX21, H2BC20P, ARHGAP11B, C1D, HLA-T
Short name

T-COR 8 Real-time PCR Thermocycler
Technique

Real-time, PCR, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment.
Alternative name

T-COR 8 Real-time PCR test kit Thermocycler
Alternative technique

rtpcr, dna-amplification

Identity

HGNC:11515
Gene

TBXT
Long gene name

T-box transcription factor T
Synonyms gene
- T
Synonyms gene name
- T, brachyury homolog (mouse)
- T brachyury transcription factor
GenBank acession
- AJ001699
Locus

6q27
Discovery year

1996-04-12
Entrez gene record

6862
Pubmed identfication
- 8963900
RefSeq identity
- NM_003181
Classification
- T-box transcription factors
VEGA ID

OTTHUMG00000015991

Identity

HGNC:7672
Gene

NCOR1
Long gene name

nuclear receptor corepressor 1
Synonyms gene name
- nuclear receptor co-repressor 1
Synonyms
- N-CoR
- hCIT529I10
- TRAC1
- hN-CoR
- KIAA1047
- MGC104216
- PPP1R109
Synonyms name
- thyroid hormone- and retinoic acid receptor-associated corepressor 1
- protein phosphatase 1, regulatory subunit 109
GenBank acession
- AF044209
Locus

17p12-p11.2
Discovery year

1999-12-17
Entrez gene record

9611
Pubmed identfication
- 7566114
- 9724795
RefSeq identity
- NM_006311
Classification
- NCoR/SMRT transcriptional repression complex subunits
- Myb/SANT domain containing
- Protein phosphatase 1 regulatory subunits
VEGA ID

OTTHUMG00000059309

Identity

HGNC:20516
Gene

H2BC20P
Long gene name

H2B clustered histone 20, pseudogene
Synonyms gene
- HIST2H2BC
Synonyms gene name
- histone 2, H2bc
- histone cluster 2, H2bc
- histone cluster 2, H2bc (pseudogene)
- histone cluster 2 H2B family member c (pseudogene)
Synonyms
- H2B/t
GenBank acession
- AY131977
Locus

1q21.2
Discovery year

2003-02-20
Entrez gene record

337873
Pubmed identfication
- 12408966
- 15527963
RefSeq identity
- NR_036461
Classification
- H2B histones
VEGA ID

OTTHUMG00000035826

Name

Polymerase Chain Reaction
Concept

Scope note: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Tree numbers
- E05.393.620.500
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data