Stem Cell Factor (human) - Clone hKL 12
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Catalog number
T-1417.0200
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Price
Please ask
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Size
200ug
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Synonym
SCF
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StorageTemperature
+5 ± 3 °C
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HOST
Mouse
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Clone
hKL 12
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Format
Purified IgG from culture supernatant, Lyophilized
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Species Crossreactivity
human
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Application
IHC, WB, ELISA
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Conjugate
NA
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Top Categories
Antibodies
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Keywords
Monoclonal; primary antibody
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Additonal Description
hKL 12 detects hSCF in tissues and isolated cells. It is useful for studying the biological effects of human stem cell factor in vitro. Synonyms for SCF are c-kit ligand, mast cell growth factor, and steel factor. Application: IHC (f, p), ELISA, Western Blot
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Description
For cells, cell lines and tissues in culture till half confluency. Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Test
Stem cell factors and stem cell growth factors will produce stem cells or be part of a transdifferentiation process to produce other cells. A cell can transdifferentiate by going back to the naive stem cell stadium or directly into the other cell, helped by the stem cell and transdifferentiationf actors. Stem cell growth factors or stem cell factors are mostly used to produce iPSCs or induced pluripotent stem cells by Jamaka or Thomson factors by using for example 5 Lenti-III-CMV viruses, expressing the Yamanaka iPSC factor set (Oct4, Sox2, Nanog and Lin28) + GFP positive control. Trans differentiation will omit the stem cell stadium but stem cell factors sill play an important role in trans differentiation strategies.
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