Recombinant Human UFM1-Activating Enzyme 5/UBA5 (N-6His)
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Catalog numberCH07-10
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Price:
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Size10 ug
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DescriptionRecombinant Human Ubiquitin-fold Modifier 1 Activating Enzyme is produced by our E.coli expression system and the target gene encoding Met1-Met404 is expressed with a 6His tag at the N-terminus.
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Species reactivityHuman
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OriginEscherichia coli
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Peptide sequenceMGSSHHHHHHSSGLVPRGSHMAESVERLQQRVQELERELAQERSLQVPRSGDGGGGRVRIEKMSSEVVDSNPYSRLMALKRMGIVSDYEKIRTFAVAIVGVGGVGSVTAEMLTRCGIGKLLLFDYDKVELANMNRLFFQPHQAGLSKVQAAEHTLRNINPDVLFEVHNYNITTVENFQHFMDRISNGGLEEGKPVDLVLSCVDNFEARMTINTACNELGQTWMESGVSENAVSGHIQLIIPGESACFACAPPLVVAANIDEKTLKREGVCAASLPTTMGVVAGILVQNVLKFLLNFGTVSFYLGYNAMQDFFPTMSMKPNPQCDDRNCRKQQEEYKKKVAALPKQEVIQEEEEIIHEDNEWGIELVSEVSEEELKNFSGPVPDLPEGITVAYTIPKKQEDSVTELTVEDSGESLEDLMAKMKNM
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Estimated molecular weight47 kDa
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Protein purityGreater than 95% as determined by reducing SDS-PAGE.
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Endotoxin levelLess than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
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Shipping conditionDry ice/ice packs
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Package formSupplied as a 0.2 µm filtered solution of 20mM TrisHCl pH 8.0,1mM DTT, 10% glycerol, 50mM NaCl.
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Storage conditionsStore at below -20°C, stable for 6 months after receipt. Please minimize freeze-thaw cycles.
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Reconstitution conditionsAlways centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 µg/ml.Dissolve the lyophilized protein in ddH2O.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
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UniProt numberQ9GZZ9
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Additional descriptionEnzymes are cleaving the substrate. If the substrate is DNA they are called restriction enzymes. Activating enzymes will cut off the domain that is biological active to become functional.
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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SourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolUFM1, UBA5
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Short nameRecombinant UFM1-Activating Enzyme 5/UBA5 (N-6His)
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TechniqueRecombinant, Enzyme, enzymes, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. novo advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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SpeciesHuman, Humans
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Alternative nameHuman UFM1 activating enzyme/UBA5(N-6His)
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Alternative techniquerec, enzymes
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Gene info
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Identity
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Gene
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Long gene nameubiquitin fold modifier 1
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Synonyms gene
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Synonyms gene name
- chromosome 13 open reading frame 20
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Synonyms
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GenBank acession
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Locus
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Discovery year2004-04-16
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameubiquitin like modifier activating enzyme 5
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Synonyms gene
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Synonyms gene name
- ubiquitin-activating enzyme E1-domain containing 1
- ubiquitin-like modifier activating enzyme 5
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2003-10-28
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Ubiquitin like modifier activating enzymes
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VEGA ID
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data