Recombinant Human Ubiquitin-Conjugating Enzyme E2 K/UBE2K (N-GST)
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Catalog numberC186-10
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PricePlease ask
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Size10 ug
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DescriptionRecombinant Human Ubiquitin-Conjugating Enzyme E2 K is produced by our E.coli expression system and the target gene encoding Met1-Asn200 is expressed with a GST tag at the N-terminus.
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Species reactivityHuman
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OriginEscherichia coli
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Peptide sequenceMSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLVPRGSMANIAVQRIKREFKEVLKSEETSKNQIKVDLVDENFTELRGEIAGPPDTPYEGGRYQLEIKIPETYPFNPPKVRFITKIWHPNISSVTGAICLDILKDQWAAAMTLRTVLLSLQALLAAAEPDDPQDAVVANQYKQNPEMFKQTARLWAHVYAGAPVSSPEYTKKIENLCAMGFDRNAVIVALSSKSWDVETATELLLSN
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Estimated molecular weight48,7 kDa
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Protein purityGreater than 95% as determined by reducing SDS-PAGE.
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Endotoxin levelLess than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
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Shipping conditionDry ice/ice packs
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Package formSupplied as a 0.2 µm filtered solution of 50mM HEPES, 150mM NaCl, 2mM DTT, 10% Glycerol, pH 7.5.
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Storage conditionsStore at below -20°C, stable for 6 months after receipt. Please minimize freeze-thaw cycles.
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Reconstitution conditionsSee included datasheet or contact us for more information.
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UniProt numberP61086
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Additional descriptionEnzymes are cleaving the substrate. If the substrate is DNA they are called restriction enzymes. Activating enzymes will cut off the domain that is biological active to become functional.
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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SourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolMGST3, GH1, UBE2K, POLM, NRAS, SDC3, PIGN, WASL, MAP6, HLA-N
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Short nameRecombinant Ubiquitin-Conjugating Enzyme E2 K/UBE2K (N-GST)
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TechniqueRecombinant, Enzyme, enzymes, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. novo advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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SpeciesHuman, Humans
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Alternative nameHuman UBE2K/E2-25K(N-GST)
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Alternative techniquerec, enzymes
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Gene info
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Identity
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Gene
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Long gene namemicrosomal glutathione S-transferase 3
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1997-10-16
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Microsomal glutathione S-transferases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namegrowth hormone 1
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2001-06-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Growth hormone family
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameubiquitin conjugating enzyme E2 K
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Synonyms gene
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Synonyms gene name
- huntingtin interacting protein 2
- ubiquitin-conjugating enzyme E2K (UBC1 homolog, yeast)
- ubiquitin-conjugating enzyme E2K
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Synonyms
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GenBank acession
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Locus
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Discovery year1997-08-28
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Ubiquitin conjugating enzymes E2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameDNA polymerase mu
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Synonyms gene name
- polymerase (DNA directed), mu
- polymerase (DNA) mu
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2000-02-16
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- DNA polymerases
- MicroRNA protein coding host genes
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameNRAS proto-oncogene, GTPase
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Synonyms gene name
- neuroblastoma RAS viral (v-ras) oncogene homolog
- neuroblastoma RAS viral oncogene homolog
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Synonyms
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GenBank acession
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Locus
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Discovery year2001-06-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- RAS type GTPase family
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namesyndecan 3
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Synonyms gene name
- syndecan 3 (N-syndecan)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1994-05-16
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Syndecans
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namephosphatidylinositol glycan anchor biosynthesis class N
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Synonyms gene name
- phosphatidylinositol glycan, class N
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-05-11
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Phosphatidylinositol glycan anchor biosynthesis
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameWASP like actin nucleation promoting factor
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Synonyms gene name
- Wiskott-Aldrich syndrome-like
- Wiskott-Aldrich syndrome like
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-10-14
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Wiskott-Aldrich Syndrome protein family
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemicrotubule associated protein 6
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-02-26
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemajor histocompatibility complex, class I, N (pseudogene)
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Synonyms
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GenBank acession
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Locus
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Discovery year2002-10-23
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Histocompatibility complex
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data