Recombinant Human Ubiquitin-Conjugating Enzyme E2 B/UBE2B/HR6B
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Catalog numberCF58-1000
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Price:
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Size1 mg
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DescriptionRecombinant Human Ubiquitin-Conjugating Enzyme E2 B is produced by our E.coli expression system and the target gene encoding Met1-Ser152 is expressed.
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Species reactivityHuman
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OriginEscherichia coli
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Peptide sequenceGSHMSTPARRRLMRDFKRLQEDPPVGVSGAPSENNIMQWNAVIFGPEGTPFEDGTFKLVIEFSEEYPNKPPTVRFLSKMFHPNVYADGSICLDILQNRWSPTYDVSSILTSIQSLLDEPNPNSPANSQAAQLYQENKREYEKRVSAIVEQSWNDS
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Estimated molecular weight17,5 kDa
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Protein purityGreater than 95% as determined by reducing SDS-PAGE.
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Endotoxin levelLess than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
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Shipping conditionDry Ice/ice packs
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Package formSupplied as a 0.2 µm filtered solution of 10mM HEPES, pH 7.5.
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Storage conditionsStore at below -20°C, stable for 6 months after receipt.Please minimize freeze-thaw cycles.
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Reconstitution conditionsAlways centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 µg/ml.Dissolve the lyophilized protein in ddH2O.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
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UniProt numberP63146
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Additional descriptionEnzymes are cleaving the substrate. If the substrate is DNA they are called restriction enzymes. Activating enzymes will cut off the domain that is biological active to become functional.
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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SourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolUBE2B
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Short nameRecombinant Ubiquitin-Conjugating Enzyme E2 B/UBE2B/HR6B
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TechniqueRecombinant, Enzyme, enzymes, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. novo advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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SpeciesHuman, Humans
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Alternative nameHuman UBE2B/HR6B
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Alternative techniquerec, enzymes
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Alternative to gene targetubiquitin-conjugating enzyme E2B, E2-17kDa and HHR6B and HR6B and RAD6B and UBC2, UBE2B and IDBG-44827 and ENSG00000119048 and 7320, ubiquitin protein ligase binding, nuclei, Ube2b and IDBG-170683 and ENSMUSG00000020390 and 22210, UBE2B and IDBG-645802 and ENSBTAG00000010982 and 282107,512207
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Gene info
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Identity
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Gene
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Long gene nameubiquitin conjugating enzyme E2 B
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Synonyms gene name
- ubiquitin-conjugating enzyme E2B (RAD6 homolog)
- ubiquitin-conjugating enzyme E2B
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Synonyms
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GenBank acession
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Locus
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Discovery year1992-12-02
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Ubiquitin conjugating enzymes E2
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VEGA ID
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data