Recombinant Human Platelet Endothelial Cell Adhesion Molecule/PECAM-1/CD31 (C-6His)
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Catalog numberCS74-10
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PricePlease ask
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Size10 ug
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DescriptionRecombinant Human Platelet Endothelial Cell Adhesion Molecule is produced by our Mammalian expression system and the target gene encoding Gln28-Lys601 is expressed with a 6His tag at the C-terminus.
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Species reactivityHuman
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OriginHuman cells
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Peptide sequenceQENSFTINSVDMKSLPDWTVQNGKNLTLQCFADVSTTSHVKPQHQMLFYKDDVLFYNISSMKSTESYFIPEVRIYDSGTYKCTVIVNNKEKTTAEYQVLVEGVPSPRVTLDKKEAIQGGIVRVNCSVPEEKAPIHFTIEKLELNEKMVKLKREKNSRDQNFVILEFPVEEQDRVLSFRCQARIISGIHMQTSESTKSELVTVTESFSTPKFHISPTGMIMEGAQLHIKCTIQVTHLAQEFPEIIIQKDKAIVAHNRHGNKAVYSVMAMVEHSGNYTCKVESSRISKVSSIVVNITELFSKPELESSFTHLDQGERLNLSCSIPGAPPANFTIQKEDTIVSQTQDFTKIASKSDSGTYICTAGIDKVVKKSNTVQIVVCEMLSQPRISYDAQFEVIKGQTIEVRCESISGTLPISYQLLKTSKVLENSTKNSNDPAVFKDNPTEDVEYQCVADNCHSHAKMLSEVLRVKVIAPVDEVQISILSSKVVESGEDIVLQCAVNEGSGPITYKFYREKEGKPFYQMTSNATQAFWTKQKANKEQEGEYYCTAFNRANHASSVPRSKILTVRVILAPWKKHHHHHH
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Estimated molecular weight65,3 kDa
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Protein purityGreater than 95% as determined by reducing SDS-PAGE.
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Endotoxin levelLess than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
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Shipping conditionAmbient/Room Temperature
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Package formLyophilized from a 0.2 µm filtered solution of PBS, pH7.4.
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Storage conditionsLyophilized protein should be stored at below -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at below -20°C for 3 months.
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Reconstitution conditionsSee included datasheet or contact us for more information.
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UniProt numberP16284
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Additional descriptioncell adhesion molecules play a role in cell growth and activation and are often identified by WB or ELISA as in the Recombinant Platelet Endothelial Adhesion Molecule/PECAM-1/CD31 (C-6His). For cells, cell lines and tissues in culture till half confluency. Whole adhesion and interacting molecules are present in lysates used as reference for ELISA quantification of these molecules and their subunits. Platelets, also called thrombocytes or cloth cells in blood and are needed to stop bleeding by clumping and clotting the blood the vessels when the an injury occurs. Teh bone marrow will produce the platelets that have no nucleus. Platelates are unique to mammals, the are curved shaped 1900nm to 3100 nm large nucleus free clothing structures.
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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SourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolPECAM1, IGHVII-1-1, MIR1-1, TRUND-NNN8-1, TRUND-NNN7-1, IGKV1OR2-1, MIR101-1, MIR1289-1, MIR16-1
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Short nameRecombinant Platelet Endothelial Adhesion Molecule/PECAM-1/CD31 (C-6His)
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. novo advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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SpeciesHuman, Humans
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Alternative nameRecombinant Human PECAM-1/CD31 (C-6His)
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Alternative techniquerec
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Tissuecell
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Gene info
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Identity
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Gene
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Long gene nameplatelet and endothelial cell adhesion molecule 1
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Synonyms gene name
- platelet/endothelial cell adhesion molecule 1
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1995-11-29
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- CD molecules
- Immunoglobulin like domain containing
- Ig-like cell adhesion molecule family
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable (II)-1-1 (pseudogene)
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Synonyms gene name
- immunoglobulin heavy variable (II)-1-1
- immunoglobulin heavy variable (II)-1-1 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-17
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemicroRNA 1-1
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Synonyms gene
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Synonyms
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Locus
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Discovery year2004-04-23
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR1/206 family
Gene info
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Identity
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Gene
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Long gene nametRNA-undetermined (NNN) 8-1
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Synonyms gene name
- transfer RNA-undetermined (NNN) 8-1
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GenBank acession
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Locus
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Discovery year2014-06-20
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Entrez gene record
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Pubmed identfication
Gene info
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Identity
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Gene
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Long gene nametRNA-undetermined (NNN) 7-1
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Synonyms gene name
- transfer RNA-undetermined (NNN) 7-1
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GenBank acession
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Locus
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Discovery year2014-06-20
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Entrez gene record
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Pubmed identfication
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 1/OR2-1 (pseudogene)
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Synonyms gene
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Synonyms gene name
- immunoglobulin kappa variable 1/OR-1
- immunoglobulin kappa variable 1/OR-1 pseudogene
- immunoglobulin kappa variable 1/OR-1 (pseudogene)
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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Pubmed identfication
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Classification
- Immunoglobulin kappa (IGK) orphons
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemicroRNA 101-1
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Synonyms gene
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Synonyms
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Locus
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Discovery year2004-04-23
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR101 family
Gene info
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Identity
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Gene
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Long gene namemicroRNA 1289-1
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Synonyms gene
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Synonyms
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Locus
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Discovery year2008-11-03
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR1289 family
Gene info
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Identity
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Gene
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Long gene namemicroRNA 16-1
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Synonyms gene
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Synonyms
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Locus
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Discovery year2004-04-23
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR15/16 family
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data