Recombinant Human NEDD8 (N-6His, SUMO tag)

• Catalog number
  CG48-10
• Price
  Please ask
• Size
  10 ug

• Description
  Recombinant Human NEDD8 is produced by our E.coli expression system and the target gene encoding Met1-Gly76 is expressed with a 6His, SUMO tag at the N-terminus.
• Species reactivity
  Human
• Origin
  Escherichia coli
• Peptide sequence
  MGHHHHHHGSLQDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLRRLMEAFAKRQGKEMDSLRFLYDGIRIQADQAPEDLDMEDNDIIEAHREQIGGMLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG
• Estimated molecular weight
  20,9 kDa
• Protein purity
  Greater than 95% as determined by reducing SDS-PAGE.
• Endotoxin level
  Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
• Shipping condition
  Ambient/Room Temperature
• Package form
  Lyophilized from a 0.2 µm filtered solution of 20mM PB,150mM NaCl,5% Trehalose,pH7.4.
• Storage conditions
  Lyophilized protein should be stored at below -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at below -20°C for 3 months.
• Reconstitution conditions
  See included datasheet or contact us for more information.
• UniProt number
  Q15843
• Other related products
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  NEDD8   N-6His   SUMO   tag  
• Gene symbol
  NEDD8-MDP1, NEDD8, SUMO1
• Short name
  Recombinant NEDD8 (N-6His, SUMO tag)
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. novo advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
• Label
  tag
• Species
  Human, Humans
• Alternative name
  Human NEDD8(N-6His, SUMO tag)
• Alternative technique
  rec

Gene info

• Identity
  HGNC:39551
• Gene
  NEDD8-MDP1
• Long gene name
  NEDD8-MDP1 readthrough
• Locus
  14q12
• Discovery year
  2011-02-21
• Entrez gene record
  100528064
• RefSeq identity
  • NM_001199823.1
• VEGA ID
  OTTHUMG00000167107

Gene info

• Identity
  HGNC:7732
• Gene
  NEDD8
• Long gene name
  NEDD8 ubiquitin like modifier
• Synonyms gene name
  • neural precursor cell expressed, developmentally down-regulated 8
• Synonyms
  • Nedd-8
• GenBank acession
  • D23662
• Locus
  14q12
• Discovery year
  1994-01-21
• Entrez gene record
  4738
• Pubmed identfication
  • 9353319
• RefSeq identity
  • NM_006156
• VEGA ID
  OTTHUMG00000029325

Gene info

• Identity
  HGNC:12502
• Gene
  SUMO1
• Long gene name
  small ubiquitin like modifier 1
• Synonyms gene
  • UBL1
• Synonyms gene name
  • ubiquitin-like 1 (sentrin)
  • SMT3 suppressor of mif two 3 homolog 1 (yeast)
  • SMT3 suppressor of mif two 3 homolog 1 (S. cerevisiae)
• Synonyms
  • PIC1
  • GMP1
  • SMT3C
  • SUMO-1
  • SMT3H3
  • OFC10
• GenBank acession
  • U38784
• Locus
  2q33.1
• Discovery year
  1996-06-04
• Entrez gene record
  7341
• Pubmed identfication
  • 8812453
  • 8906799
• RefSeq identity
  • NM_003352
• VEGA ID
  OTTHUMG00000132839

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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