Recombinant Human NEDD8-Conjugating Enzyme UBC12/UBE2M

• Catalog number
  CG49-50
• Price
  Please ask
• Size
  50 ug

• Description
  Recombinant Human Ubiquitin-conjugating Enzyme E2M is produced by our E.coli expression system and the target gene encoding Met1-Lys183 is expressed.
• Species reactivity
  Human
• Origin
  Escherichia coli
• Peptide sequence
  MIKLFSLKQQKKEEESAGGTKGSSKKASAAQLRIQKDINELNLPKTCDISFSDPDDLLNFKLVICPDEGFYKSGKFVFSFKVGQGYPHDPPKVKCETMVYHPNIDLEGNVCLNILREDWKPVLTINSIIYGLQYLFLEPNPEDPLNKEAAEVLQNNRRLFEQNVQRSMRGGYIGSTYFERCLK
• Estimated molecular weight
  20,9 kDa
• Protein purity
  Greater than 95% as determined by reducing SDS-PAGE.
• Endotoxin level
  Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
• Shipping condition
  Dry ice/ice packs
• Package form
  Supplied as a 0.2 µm filtered solution of 50mM HEPES,pH 7.5,2mM DTT,150mM NaCl,10%glycerol .
• Storage conditions
  Store at below -20°C, stable for 6 months after receipt. Please minimize freeze-thaw cycles.
• Reconstitution conditions
  See included datasheet or contact us for more information.
• UniProt number
  P61081
• Other related products
• Additional description
  Enzymes are cleaving the substrate. If the substrate is DNA they are called restriction enzymes. Activating enzymes will cut off the domain that is biological active to become functional.
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  NEDD8-Conjugating   UBC12/UBE2M  
• Gene symbol
  NEDD8-MDP1, UBE2M, NEDD8
• Short name
  Recombinant NEDD8-Conjugating Enzyme UBC12/UBE2M
• Technique
  Recombinant, Enzyme, enzymes, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. novo advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  Human UBE2M/Ubc12
• Alternative technique
  rec, enzymes

Gene info

• Identity
  HGNC:39551
• Gene
  NEDD8-MDP1
• Long gene name
  NEDD8-MDP1 readthrough
• Locus
  14q12
• Discovery year
  2011-02-21
• Entrez gene record
  100528064
• RefSeq identity
  • NM_001199823.1
• VEGA ID
  OTTHUMG00000167107

Gene info

• Identity
  HGNC:12491
• Gene
  UBE2M
• Long gene name
  ubiquitin conjugating enzyme E2 M
• Synonyms gene name
  • ubiquitin-conjugating enzyme E2M (homologous to yeast UBC12)
  • ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast)
  • ubiquitin-conjugating enzyme E2M
• Synonyms
  • hUbc12
  • UBC12
• GenBank acession
  • AB012191
• Locus
  19q13.43
• Discovery year
  1999-01-22
• Entrez gene record
  9040
• Pubmed identfication
  • 9694792
• RefSeq identity
  • NM_003969
• Classification
  • Ubiquitin conjugating enzymes E2
• VEGA ID
  OTTHUMG00000183548

Gene info

• Identity
  HGNC:7732
• Gene
  NEDD8
• Long gene name
  NEDD8 ubiquitin like modifier
• Synonyms gene name
  • neural precursor cell expressed, developmentally down-regulated 8
• Synonyms
  • Nedd-8
• GenBank acession
  • D23662
• Locus
  14q12
• Discovery year
  1994-01-21
• Entrez gene record
  4738
• Pubmed identfication
  • 9353319
• RefSeq identity
  • NM_006156
• VEGA ID
  OTTHUMG00000029325

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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