Recombinant Human Matrix Metalloproteinase-9/MMP-9 (C-6His)
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Catalog numberCI71-500
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PricePlease ask
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Size500 ug
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DescriptionRecombinant Human Matrix metalloproteinase-9 is produced by our Mammalian expression system and the target gene encoding Ala19-Asp707 is expressed with a 6His tag at the C-terminus.
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Species reactivityHuman
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OriginHuman cells
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Peptide sequenceAAPRQRQSTLVLFPGDLRTNLTDRQLAEEYLYRYGYTRVAEMRGESKSLGPALLLLQKQLSLPETGELDSATLKAMRTPRCGVPDLGRFQTFEGDLKWHHHNITYWIQNYSEDLPRAVIDDAFARAFALWSAVTPLTFTRVYSRDADIVIQFGVAEHGDGYPFDGKDGLLAHAFPPGPGIQGDAHFDDDELWSLGKGVVVPTRFGNADGAACHFPFIFEGRSYSACTTDGRSDGLPWCSTTANYDTDDRFGFCPSERLYTRDGNADGKPCQFPFIFQGQSYSACTTDGRSDGYRWCATTANYDRDKLFGFCPTRADSTVMGGNSAGELCVFPFTFLGKEYSTCTSEGRGDGRLWCATTSNFDSDKKWGFCPDQGYSLFLVAAHEFGHALGLDHSSVPEALMYPMYRFTEGPPLHKDDVNGIRHLYGPRPEPEPRPPTTTTPQPTAPPTVCPTGPPTVHPSERPTAGPTGPPSAGPTGPPTAGPSTATTVPLSPVDDACNVNIFDAIAEIGNQLYLFKDGKYWRFSEGRGSRPQGPFLIADKWPALPRKLDSVFEEPLSKKLFFFSGRQVWVYTGASVLGPRRLDKLGLGADVAQVTGALRSGRGKMLLFSGRRLWRFDVKAQMVDPRSASEVDRMFPGVPLDTHDVFQYREKAYFCQDRFYWRVSSRSELNQVDQVGYVTYDILQCPEDVDHHHHHH
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Estimated molecular weight77,4 kDa
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Protein purityGreater than 95% as determined by reducing SDS-PAGE.
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Endotoxin levelLess than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
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Shipping conditionDry Ice/ice packs
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Package formSupplied as a 0.2 µm filtered solution of 20mM TrisHCl, 2mM CaCl2, 150mM NaCl, 0.05% Brij35(w/v), pH 7.5.
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Storage conditionsStore at below -20°C, stable for 6 months after receipt. Please minimize freeze-thaw cycles.
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Reconstitution conditionsAlways centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 µg/ml.Dissolve the lyophilized protein in ddH2O.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
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UniProt numberP14780
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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SourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolMMP9, MMP14, MMP24, MMP28, RNU6-9, PIRC47, PIRC38, PIRC43, PIRC45
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Short nameRecombinant Matrix Metalloproteinase-9/MMP-9 (C-6His)
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. novo advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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SpeciesHuman, Humans
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Alternative nameHuman MMP-9(C-6His)
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Alternative techniquerec
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Gene info
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Identity
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Gene
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Long gene namematrix metallopeptidase 9
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Synonyms gene
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Synonyms gene name
- matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
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Locus
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Discovery year1990-03-14
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Entrez gene record
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Pubmed identfication
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Classification
- M10 matrix metallopeptidases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namematrix metallopeptidase 14
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Synonyms gene name
- matrix metalloproteinase 14 (membrane-inserted)
- matrix metallopeptidase 14 (membrane-inserted)
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Synonyms
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Synonyms name
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Locus
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Discovery year1994-11-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- M10 matrix metallopeptidases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namematrix metallopeptidase 24
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Synonyms gene name
- matrix metalloproteinase 24 (membrane-inserted)
- matrix metallopeptidase 24 (membrane-inserted)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1999-08-06
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- M10 matrix metallopeptidases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namematrix metallopeptidase 28
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Synonyms gene name
- matrix metalloproteinase 28
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Synonyms
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GenBank acession
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Locus
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Discovery year2001-01-09
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- M10 matrix metallopeptidases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameRNA, U6 small nuclear 9
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Synonyms
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Locus
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Discovery year2008-06-10
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nuclear RNAs
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 47
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 38
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 43
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 45
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data