Recombinant Human GM-CSF/CSF2 (E. coli)
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Catalog numberC003-1000
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Price:
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Size1 mg
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DescriptionRecombinant Human Granulocyte-Macrophage Colony-Stimulating Factor is produced by our E.coli expression system and the target gene encoding Ala18-Glu144 is expressed.
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Species reactivityHuman
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OriginEscherichia coli
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Peptide sequenceMAPARSPSPSTQPWEHVNAIQEARRLLNLSRDTAAEMNETVEVISEMFDLQEPTCLQTRLELYKQGLRGSLTKLKGPLTMMASHYKQHCPPTPETSCATQIITFESFKENLKDFLLVIPFDCWEPVQE
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Estimated molecular weight14,6 kDa
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Protein purityGreater than 95% as determined by reducing SDS-PAGE.
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Endotoxin levelLess than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
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Shipping conditionAmbient/Room Temperature
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Package formLyophilized from a 0.2 µm filtered solution of 20mM PB, 150mM NaCl, 5% Mannitol, pH 7.2.
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Storage conditionsLyophilized protein should be stored at below -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at below -20°C for 3 months.
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Reconstitution conditionsSee included datasheet or contact us for more information.
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UniProt numberP04141
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GeneGranulocyte-macrophage colony-stimulating factor (GM-CSF), also known as colony stimulating factor 2 (CSF2), is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, NK cells, endothelial cells and fibroblasts that functions as a cytokine. The pharmaceutical analogs of naturally occurring GM-CSF are called sargramostim and molgramostim. Reconstitution It is recommended that sterile ddH2O containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of not less than 1 mg/mL of the cytokine.
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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SourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolCSF2, PPP1R3A, CSF1, CSF3, IL3
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Short nameRecombinant GM-CSF/CSF2 ( )
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. novo advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Hoste. coli, Escherichia coli, Recombination of bioactive proteins and longer peptides in Escherichia Coli is done often with His tagging. novo supplies Rec. E. Coli affinity purified or tag purified antigens in 1 quantities or bulk volumes on request.
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SpeciesE. coli, E. coli, Humans
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Alternative nameHuman GM-CSF
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Alternative techniquerec, escherichia
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Alternative to gene targetcolony stimulating factor 2 (granulocyte-macrophage), GMCSF, CSF2 and IDBG-41275 and ENSG00000164400 and 1437, growth factor activity, Extracellular, Csf2 and IDBG-172875 and ENSMUSG00000018916 and 12981, CSF2 and IDBG-644701 and ENSBTAG00000001570 and 281095
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Gene info
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Identity
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Gene
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Long gene namecolony stimulating factor 2
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Synonyms gene name
- colony stimulating factor 2 (granulocyte-macrophage)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2001-06-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameprotein phosphatase 1 regulatory subunit 3A
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Synonyms gene
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Synonyms gene name
- protein phosphatase 1, regulatory (inhibitor) subunit 3A (glycogen and sarcoplasmic reticulum binding subunit, skeletal muscle)
- protein phosphatase 1, regulatory (inhibitor) subunit 3A
- protein phosphatase 1, regulatory subunit 3A
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1993-01-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Protein phosphatase 1 regulatory subunits
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namecolony stimulating factor 1
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Synonyms gene name
- colony stimulating factor 1 (macrophage)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1986-01-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namecolony stimulating factor 3
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Synonyms gene
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Synonyms gene name
- chromosome 17 open reading frame 33
- colony stimulating factor 3 (granulocyte)
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Synonyms
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Synonyms name
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Locus
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Discovery year2001-06-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Receptor ligands
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameinterleukin 3
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Synonyms gene name
- interleukin 3 (colony-stimulating factor, multiple)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2001-06-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Interleukins
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VEGA ID
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data