Recombinant Human DNA Fragmentation Factor Subunit a/DFFA/DFF45/ICAD (N-6His)

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• Catalog number
  CE91-1000
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• Size
  1 mg

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• Description
  Recombinant Human DFF45 is produced by our E.coli expression system and the target gene encoding Met1-Thr331 is expressed with a 6His tag at the N-terminus.
• Species reactivity
  Human
• Origin
  Escherichia coli
• Peptide sequence
  MGSSHHHHHHSSGLVPRGSHMEVTGDAGVPESGEIRTLKPCLLRRNYSREQHGVAASCLEDLRSKACDILAIDKSLTPVTLVLAEDGTIVDDDDYFLCLPSNTKFVALASNEKWAYNNSDGGTAWISQESFDVDETDSGAGLKWKNVARQLKEDLSSIILLSEEDLQMLVDAPCSDLAQELRQSCATVQRLQHTLQQVLDQREEVRQSKQLLQLYLQALEKEGSLLSKQEESKAAFGEEVDAVDTGISRETSSDVALASHILTALREKQAPELSLSSQDLELVTKEDPKALAVALNWDIKKTETVQEACEWELALRLQQTQSLHSLRSISASKASPPGDLQNPKRARQDPT
• Estimated molecular weight
  38,7 kDa
• Protein purity
  Greater than 95% as determined by reducing SDS-PAGE.
• Endotoxin level
  Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
• Shipping condition
  Dry Ice/ice packs
• Package form
  Supplied as a 0.2 µm filtered solution of 20mM PB,150mM NaCl,pH7.4.
• Storage conditions
  Store at below -20°C, stable for 6 months after receipt.Please minimize freeze-thaw cycles.
• Reconstitution conditions
  Always centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 µg/ml.Dissolve the lyophilized protein in ddH2O.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• UniProt number
  O00273
• Other related products
• Additional description
  Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Source
  Recombinants or rec. proteins
• Group
  recombinants

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• Gene target
  DNA   Fragmentation   Factor   Subunit   a/DFFA/DFF45/ICAD   N-6His  
• Gene symbol
  DFFA, PRKDC, HLA-DOA
• Short name
  Recombinant DNA Fragmentation Factor Subunit a/DFFA/DFF45/ICAD (N-6His)
• Technique
  Recombinant, dna, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. novo advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  Human DFF45/DFFA/ICAD(N-6His)
• Alternative technique
  rec
• Alternative to gene target
  DNA fragmentation factor, 45kDa, alpha polypeptide, DFF-45 and DFF1 and ICAD, DFFA and IDBG-89047 and ENSG00000160049 and 1676, protein binding, nuclei, Dffa and IDBG-204894 and ENSMUSG00000028974 and 13347

Gene info

• Identity
  HGNC:2772
• Gene
  DFFA
• Long gene name
  DNA fragmentation factor subunit alpha
• Synonyms gene name
  • DNA fragmentation factor, 45 kD, alpha polypeptide
  • DNA fragmentation factor, 45kDa, alpha polypeptide
• Synonyms
  • DFF-45
  • DFF45
  • ICAD
  • DFF1
• Synonyms name
  • DNA fragmentation factor, 45 kD, alpha subunit
• GenBank acession
  • AF087573
• Locus
  1p36.22
• Discovery year
  1997-10-27
• Entrez gene record
  1676
• Pubmed identfication
  • 9605855
  • 9108473
• RefSeq identity
  • NM_004401
• VEGA ID
  OTTHUMG00000001909

Gene info

• Identity
  HGNC:9413
• Gene
  PRKDC
• Long gene name
  protein kinase, DNA-activated, catalytic subunit
• Synonyms gene
  • HYRC
  • HYRC1
• Synonyms gene name
  • protein kinase, DNA-activated, catalytic polypeptide
• Synonyms
  • DNPK1
  • p350
  • DNAPK
  • XRCC7
  • DNA-PKcs
  • DNAPKc
  • DNA-PKC
  • p460
• Synonyms name
  • DNA-dependent protein kinase catalytic subunit
• Locus
  8q11.21
• Discovery year
  1993-11-09
• Entrez gene record
  5591
• Pubmed identfication
  • 7638222
• RefSeq identity
  • NM_001081640
• Classification
  • Armadillo like helical domain containing
• VEGA ID
  OTTHUMG00000164239
• Locus Specific Databases
  • LRG_162

Gene info

• Identity
  HGNC:4936
• Gene
  HLA-DOA
• Long gene name
  major histocompatibility complex, class II, DO alpha
• Synonyms gene
  • HLA-DZA
  • HLA-DNA
• Synonyms
  • HLA-D0-alpha
• GenBank acession
  • M31525
• Locus
  6p21.32
• Discovery year
  2001-06-22
• Entrez gene record
  3111
• Pubmed identfication
  • 2370084
• RefSeq identity
  • NM_002119
• Classification
  • C1-set domain containing
  • Histocompatibility complex
• VEGA ID
  OTTHUMG00000031211
• Locus Specific Databases
  • IMGT/HLA Database

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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