Recombinant Human DNA Fragmentation Factor Subunit a/DFFA/DFF45/ICAD (N-6His)
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Catalog numberCE91-1000
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Price:
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Size1 mg
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DescriptionRecombinant Human DFF45 is produced by our E.coli expression system and the target gene encoding Met1-Thr331 is expressed with a 6His tag at the N-terminus.
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Species reactivityHuman
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OriginEscherichia coli
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Peptide sequenceMGSSHHHHHHSSGLVPRGSHMEVTGDAGVPESGEIRTLKPCLLRRNYSREQHGVAASCLEDLRSKACDILAIDKSLTPVTLVLAEDGTIVDDDDYFLCLPSNTKFVALASNEKWAYNNSDGGTAWISQESFDVDETDSGAGLKWKNVARQLKEDLSSIILLSEEDLQMLVDAPCSDLAQELRQSCATVQRLQHTLQQVLDQREEVRQSKQLLQLYLQALEKEGSLLSKQEESKAAFGEEVDAVDTGISRETSSDVALASHILTALREKQAPELSLSSQDLELVTKEDPKALAVALNWDIKKTETVQEACEWELALRLQQTQSLHSLRSISASKASPPGDLQNPKRARQDPT
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Estimated molecular weight38,7 kDa
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Protein purityGreater than 95% as determined by reducing SDS-PAGE.
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Endotoxin levelLess than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
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Shipping conditionDry Ice/ice packs
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Package formSupplied as a 0.2 µm filtered solution of 20mM PB,150mM NaCl,pH7.4.
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Storage conditionsStore at below -20°C, stable for 6 months after receipt.Please minimize freeze-thaw cycles.
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Reconstitution conditionsAlways centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 µg/ml.Dissolve the lyophilized protein in ddH2O.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
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UniProt numberO00273
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Additional descriptionAplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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SourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolDFFA, PRKDC, HLA-DOA
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Short nameRecombinant DNA Fragmentation Factor Subunit a/DFFA/DFF45/ICAD (N-6His)
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TechniqueRecombinant, dna, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. novo advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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SpeciesHuman, Humans
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Alternative nameHuman DFF45/DFFA/ICAD(N-6His)
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Alternative techniquerec
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Alternative to gene targetDNA fragmentation factor, 45kDa, alpha polypeptide, DFF-45 and DFF1 and ICAD, DFFA and IDBG-89047 and ENSG00000160049 and 1676, protein binding, nuclei, Dffa and IDBG-204894 and ENSMUSG00000028974 and 13347
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Gene info
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Identity
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Gene
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Long gene nameDNA fragmentation factor subunit alpha
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Synonyms gene name
- DNA fragmentation factor, 45 kD, alpha polypeptide
- DNA fragmentation factor, 45kDa, alpha polypeptide
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1997-10-27
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameprotein kinase, DNA-activated, catalytic subunit
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Synonyms gene
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Synonyms gene name
- protein kinase, DNA-activated, catalytic polypeptide
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Synonyms
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Synonyms name
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Locus
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Discovery year1993-11-09
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Armadillo like helical domain containing
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namemajor histocompatibility complex, class II, DO alpha
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Synonyms gene
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Synonyms
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GenBank acession
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Locus
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Discovery year2001-06-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- C1-set domain containing
- Histocompatibility complex
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data