Recombinant Human Caspase-10/CASP10 (C-6His)
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Catalog number
CF93-10
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Price
Please ask
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Size
10 ug
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Description
Recombinant Human Caspase-10 is produced by our E.coli expression system and the target gene encoding Val220-Ile480 is expressed with a 6His tag at the C-terminus.
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Species reactivity
Human
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Origin
Escherichia coli
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Peptide sequence
MVKTFLEALPQESWQNKHAGSNGNRATNGAPSLVSRGMQGASANTLNSETSTKRAAVYRMNRNHRGLCVIVNNHSFTSLKDRQGTHKDAEILSHVFQWLGFTVHIHNNVTKVEMEMVLQKQKCNPAHADGDCFVFCILTHGRFGAVYSSDEALIPIREIMSHFTALQCPRLAEKPKLFFIQACQGEEIQPSVSIEADALNPEQAPTSLQDSIPAEADFLLGLATVPGYVSFRHVEEGSWYIQSLCNHLKKLVPRHEDILSILEHHHHHH
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Estimated molecular weight
30,1 kDa
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Protein purity
Greater than 95% as determined by reducing SDS-PAGE.
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Endotoxin level
Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
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Shipping condition
Dry Ice/ice packs
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Package form
Supplied as a 0.2 µm filtered solution of 25mM HEPES, 10mM DTT, pH 7.5.
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Storage conditions
Store at below -20°C, stable for 6 months after receipt. Please minimize freeze-thaw cycles.
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Reconstitution conditions
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 µg/ml.Dissolve the lyophilized protein in ddH2O.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
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UniProt number
Q92851-2
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Additional description
Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
CASP10
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Short name
Recombinant Caspase-10/CASP10 (C-6His)
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. novo advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Species
Human, Humans
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Alternative name
Human Caspase-10/CASP10(C-6His)
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Alternative technique
rec
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Alternative to gene target
caspase 10, apoptosis-related cysteine peptidase, ALPS2 and FLICE2 and MCH4, CASP10 and IDBG-78470 and ENSG00000003400 and 843, cysteine-type endopeptidase activity involved in apoptotic signaling pathway, Plasma membranes
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Gene info
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Identity
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Gene
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Long gene name
caspase 10
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Synonyms gene name
- caspase 10, apoptosis-related cysteine protease
- caspase 10, apoptosis-related cysteine peptidase
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year
1997-04-21
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Caspases
- Death effector domain containing
- Death inducing signaling complex
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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