Recombinant E. coli N-Acetyl-D-Glucosamine Kinase/NAGK (C-6His)
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Catalog number
C666-1000
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Price
Please ask
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Size
1 mg
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Description
Recombinant E.coli N-acetyl-D-Glucosamine Kinase is produced by our E.coli expression system and the target gene encoding Met1-Asp303 is expressed with a 6His tag at the C-terminus.
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Species reactivity
E.coli
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Origin
Escherichia coli
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Peptide sequence
MNHKVHHHHHHMYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVCELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRLDNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRLPVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQGDEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPVARVPRIERARHGDAGGMRGAAFLHLTD
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Estimated molecular weight
34,5 kDa
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Protein purity
Greater than 95% as determined by reducing SDS-PAGE.
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Endotoxin level
Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
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Shipping condition
Dry ice/ice packs
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Package form
Supplied as a 0.2 µm filtered solution of 20mM TrisHCl, 100mM NaCl,2mM DTT,20% Glycerol, pH 8.0.
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Storage conditions
Store at below -20°C, stable for 6 months after receipt.Please minimize freeze-thaw cycles.
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Reconstitution conditions
See included datasheet or contact us for more information.
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UniProt number
P75959
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Additional description
N terminal acetylation or CH3CO as epigenetic regulation of Recombinant N- -D-Glucosamine Kinase/NAGK (C-6His) by NATs.
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Source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
NAGK
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Short name
Recombinant N- -D-Glucosamine Kinase/NAGK (C-6His)
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. novo advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Host
e. coli, Escherichia coli, Recombination of bioactive proteins and longer peptides in Escherichia Coli is done often with His tagging. novo supplies Rec. E. Coli affinity purified or tag purified antigens in 1 quantities or bulk volumes on request.
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Species
E. coli, E. coli
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Alternative name
Recombinant E.coli NAGK(C-6His)
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Alternative technique
rec, escherichia
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Gene info
MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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