Recombinant E. coli Methionine Aminopeptidase/MetAP/MAP (C-6His)

• Catalog number
  C151-1000
• Price
  Please ask
• Size
  1 mg

• Description
  Recombinant E.coli Methionine Aminopeptidase is produced by our E.coli expression system and the target gene encoding Ala2-Glu264 is expressed with a 6His tag at the C-terminus.
• Species reactivity
  E.coli
• Origin
  Escherichia coli
• Peptide sequence
  AISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRICNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIKDGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAEGFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMKDGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDELEHHHHHH
• Estimated molecular weight
  30,4 kDa
• Protein purity
  Greater than 95% as determined by reducing SDS-PAGE.
• Endotoxin level
  Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
• Shipping condition
  Dry ice/ice packs
• Package form
  Supplied as a 0.2 µm filtered solution of 20mM TrisHCl, 100mM NaCl, 50% Glycerol, pH 8.0.
• Storage conditions
  Store at below -20°C, stable for 6 months after receipt.Please minimize freeze-thaw cycles.
• Reconstitution conditions
  Always centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 µg/ml.Dissolve the lyophilized protein in ddH2O.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• UniProt number
  P0AE18
• Other related products
• Source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Methionine   Aminopeptidase/MetAP/MAP   C-6His  
• Gene symbol
  MOAP1, MAP7, MASP2, MASP1
• Short name
  Recombinant Methionine Aminopeptidase/MetAP/MAP (C-6His)
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. novo advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Host
  e. coli, Escherichia coli, Recombination of bioactive proteins and longer peptides in Escherichia Coli is done often with His tagging. novo supplies Rec. E. Coli affinity purified or tag purified antigens in 1 quantities or bulk volumes on request.
• Species
  E. coli, E. coli
• Alternative name
  E.coli MetAP/MAP(C-6His)
• Alternative technique
  rec, escherichia

Gene info

• Identity
  HGNC:16658
• Gene
  MOAP1
• Long gene name
  modulator of apoptosis 1
• Synonyms
  • MAP-1
  • PNMA4
• Synonyms name
  • paraneoplastic Ma antigen family member 4
• GenBank acession
  • BC015044
• Locus
  14q32.12
• Discovery year
  2002-11-13
• Entrez gene record
  64112
• Pubmed identfication
  • 11060313
• Classification
  • Paraneoplastic Ma antigens
• VEGA ID
  OTTHUMG00000169184

Gene info

• Identity
  HGNC:6869
• Gene
  MAP7
• Long gene name
  microtubule associated protein 7
• Synonyms
  • E-MAP-115
• Synonyms name
  • ensconsin
• GenBank acession
  • X73882
• Locus
  6q23.3
• Discovery year
  1999-01-22
• Entrez gene record
  9053
• Pubmed identfication
  • 8408219
• RefSeq identity
  • NM_003980
• VEGA ID
  OTTHUMG00000015646

Gene info

• Identity
  HGNC:6902
• Gene
  MASP2
• Long gene name
  MBL associated serine protease 2
• Synonyms gene
  • MASP1P1
• Synonyms gene name
  • mannan-binding lectin serine protease 2
  • mannan-binding lectin serine peptidase 1 pseudogene 1
  • mannan-binding lectin serine protease 1 pseudogene 1
• Synonyms
  • Map19
  • sMAP
  • MAP-2
• Synonyms name
  • mannose-binding lectin-associated serine protease 2
  • mannose-binding lectin associated protein 19
• GenBank acession
  • X98400
• Locus
  1p36.22
• Discovery year
  1998-12-17
• Entrez gene record
  10747
• Pubmed identfication
  • 9087411
  • 9777418
• RefSeq identity
  • NM_006610
• Classification
  • Sushi domain containing
  • Complement system activation components
• VEGA ID
  OTTHUMG00000002121
• Locus Specific Databases
  • MASP2base: Mutation registry for MASP-2 deficiency
  • LRG_82

Gene info

• Identity
  HGNC:6901
• Gene
  MASP1
• Long gene name
  MBL associated serine protease 1
• Synonyms gene
  • CRARF
  • PRSS5
• Synonyms gene name
  • mannan-binding lectin serine protease 1 (C4/C2 activating component of Ra-reactive factor)
  • mannan-binding lectin serine peptidase 1 (C4/C2 activating component of Ra-reactive factor)
  • mannan binding lectin serine peptidase 1
• Synonyms
  • MASP
  • Map44
  • MASP-3
  • MAP-1
• Synonyms name
  • C4/C2 activating component of Ra-reactive factor
  • mannose-binding lectin-associated serine protease 1
• GenBank acession
  • D28593
• Locus
  3q27.3
• Discovery year
  1995-07-06
• Entrez gene record
  5648
• Pubmed identfication
  • 8018603
  • 8240317
• RefSeq identity
  • NM_001879
• Classification
  • Serine proteases
  • Complement system activation components
  • Sushi domain containing
• VEGA ID
  OTTHUMG00000156461
• Locus Specific Databases
  • LRG_349

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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