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Category
Primary Antibodies
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Long description
The defined antibody specificity is to the hepatitis associated antigen as tested in counterelectrophoresis and double radial immunodiffusion against HAA positive human serum. The product does not react with normal human serum or plasma. In precipitating techniques as counteroelectrophoresis and double radial immunodiffusion (Ouchterlony) to identify the presence of hepatitis associated antigen.
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Antibody come from
The immunogen has been prepared from sera of patients with hepatitis using gel filtration Freund’s complete adjuvant is used in the first step of the immunization procedure.
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Other description
Delipidated, heat inactivated, lyophilized, stable whole serum. No preservative added. Total protein and IgG concentrations in the antiserum are comparable to those of normal pooled swine serum. No foreign proteins added.
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Clone
Polyclonal
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Antigen antibody binding interaction
Swine anti Human Hepatitis B associated antigen Antibody
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Antibody is raised in
Swine
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Antibody s reacts with
Human
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Antibody s reacts with these species
Inter-species cross-reactivity of this antiSerum has not been tested in detail.
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Antibody s specificity
Precipitating polyclonal Swine antiSerum to Human hepatitis associated antigen.
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Research interest
Microbiology
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Application
Immunoprecipitation
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Antibody s suited for
Immunoprecipitation. In counter electrophoresis use opposite holes at a distance of 4mm with 6 μl antiserum and serum samples. In double radial immunodiffusion use a rosette arrangement with 10 μl antiserum in 3 mm diameter center well and 2 μl plasma samples (neat and serially diluted) in 2 mm diameter peripheral wells. The distance between the wells should be 2-3 mm. In case the concentration of the protein component is significantly increased or reduced, the required equivalence between the reactants can be establish by increasing the amount of antiserum or antigen respectively.
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Storage
The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day. Lyophilized at +4°C--at least 10 years. Reconstituted at or below -20°C--3-5 years. Reconstituted at +4°C--7 days.
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Relevant references
no information yet
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk. Antigens are peptides or recombinant or native dependent on the production method. Hepatitis a, b, c, d, e recombinant surface viral antigens to genotype 1, 2, 3, 4, 5 are used for antibody production to make diagnostic poly or monoclonal antibodies. Hepatitis is a disease of the liver characterized by the presence of inflammatory cells in the tissue of the organ. Hepatitis may occur without symptoms, but can lead to jaundice (a yellow discoloration of the skin, mucous membranes, and conjunctiva of the eyes), poor appetite, and fatigue. Depending on the cause, hepatitis can manifest either as an acute or as a chronic disease.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
