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Category
Proteins & Peptides
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Long description
Rat sphingosine kinase 1 catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (spp)(sphingosine + ATP = sphingosine 1-phosphate + ADP), a lipid mediator with both intra- and extracellular functions. also acts on d-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as d,l-threo-dihydrosphingosine, n,n-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol. The long form of sphingosine kinase 1, an N-terminal extension, is responsible for FceRI-mediated calcium mobilization and may play a role in other ligand mediated signaling events.
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Antibody come from
Recombinant protein corresponding to amino acid residues 853-1052 from the C-terminal region of human FAK.
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Other description
Provided as solution in phosphate buffered saline, pH 7.3, with 0.2%l BSA and 0.01% sodium azide.
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Clone
not specified
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Antigen antibody binding interaction
Sphingosine Kinase 2 antibody Long Form antibody Human - Blocking Peptide
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Antibody is raised in
Rabbit
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Antibody s reacts with
see techfile
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Application
Antibody function blocking
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Antibody s suited for
Dilutions: For immunoprecipitation, the recommended concentration is 10 µg/mg of protein lysate; and for Western blot analysis, the recommended concentration is 5 - 10 µg/mL with an incubation for 2 hours at room temperature. Optimal concentration should be evaluated by serial dilutions.
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Storage
-20ºC
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Relevant references
1) Akiyama, S.K. (1996) Integrins in cell adhesion and signaling. Hum. Cell 9(3):181-186. 2) Cance, W.G. and E.T. Liu (1995) Protein kinases in human breast cancer. Breast Cancer Res. Treat. 35(1):105-114. _x000B_3) Hanks, S.K. and T.R. Polte (1997) Signaling through focal adhesion kinase. BioEssays 19(2):137-145. _x000B_4) Ilic, D., C.H. Damsky, and T. Yamamoto (1997) Focal adhesion kinase: at the crossroads of signal transduction. J. Cell Sci. 110:401-407. _x000B_5) Martin, G.M. (1996) Fak and focal adhesions. Jpn. J. Cancer Res. 87(3):1. _x000B_6) Morimoto, C. and K. Tachibana (1996) Beta 1 integrin-mediated signaling in human T- cells. Hum. Cell 9(3):163-168. _x000B_7) Neet, K. and T. Hunter (1996) Vertebrate non-receptor protein-tyrosine kinase families. Genes Cells 1(2):147-169. _x000B_8) Otey, C.A. (1996) pp125FAK in focal adhesion. Internat. Rev. Cytol. 167:161-183. _x000B_9) Owens, L.V., L. Xu, R.J. Craven, G.A. Dent, T.M. Weiner, L. Kornberg, E.T. Liu, and W.G. Cance (1995) Overexpression of the focal adhesion kinase (p125FAK) in invasive human tumors. Cancer Res. 55(13):2752-2755. _x000B_10) Schlaepfer, D.D. and T. Hunter (1996) Signal transduction from the extracellular matrixÑa role for the focal adhesion protein-tyrosine kinase FAK. Cell Struct. Funct. 21(5):445-450.
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Properties
If you buy Antibodies supplied by nordc they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. blocking peptide, Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Test
You can block the antibody by the specific target amino acid sequence of peptide.
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Description
Peptides short amino acid chains or epitopes or blocking antagonists. The shortest peptides are dipeptides, consisting of 2 amino acids joined by a single peptide bond, followed by tripeptides, tetra peptides, ... till polypeptides that are long, continuous, and unbranched synthetic peptide chains. These biological oligomers and polymers can be Solid-phase peptide synthesis (SPPS), or in continue produced for custom peptide synthesis projects. The High-efficiency solid phase peptide synthesis (HE-SPPS) is give very low production costs.
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French translation
anticorps
