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Category
Proteins & Peptides
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Long description
SPARC is a key factor in cell-matrix interactions and possibly tumour aggressiveness. The SPARC gene, which encodes a multifunctional glycoprotein with roles in tissue development, remodelling and _x000B_fibrosis. A regulator of cell-extracellular matrix (ECM) interactions, SPARC represents a major factor in the ECM remodelling occurring during tumour invasion. in silico analysis reveals 4 UTR-SNPs located in the 3 -UTR of the SPARC gene, corresponding to 1474 g a, 1551 g c, 1922 t g and 2072 c t changes, which are significantly associated with tumoral state of the tissue. Of all hits, the 2072 SPARC polymorphism had the best association with cancer._x000B__x000B_SPARC therefore is a gene involved in a number of diseases including rheumatoid arthritis, scleroderma, tumor development and metastasis. SPARC variants have been detected in tumour samples of patients with acute myeloblastic leukemia (AML).
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Antibody come from
Synthetic peptide derived from the human Netrin 1 protein
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Other description
Provided as ligand affinity purified antibody in phosphate buffered saline with 0.08% sodium azide.
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Clone
not specified
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Antigen antibody binding interaction
SPARC - Blocking Peptide
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Antibody is raised in
see techfile
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Antibody s reacts with
Human
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Application
Antibody function blocking
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Antibody s suited for
Antibody can be used for Western blotting (0.05-0.5 µg/ml). Optimal concentration should be evaluated by serial dilutions.
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Storage
-20ºC
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Relevant references
1. Nguyen, A., et al. 'Netrin-1 induces angiogenesis via a DCC-dependent ERK 1/2-eNOS feed-forward mechanism.' Proc. Natl. Acad. Sci. USA, 2006, 103, 6530-6535_x000B__x000B_2. Shekarabi, M., et al. 'Deleted in colorectal cancer binding netrin-1 mediates cell substrate adhesion and recruits Cdc42, Rac1, Pak1, and N-WASP into an intracellular signaling comlex that promotes growth cone expansion.' J. Neurosci. 2005, 25, 3132-3141_x000B__x000B_3. Jarjour, A.A., et al. 'Netrin-1 is a chemorepellent for oligodendrocyte precursor cells in the embryonic spinal cord.' J. Neurosci. 2003, 23, 3735-3744_x000B__x000B_4. Furne C, et al. 'The dependence receptor DCC requires lipid raft localization for cell death signaling.' Proc Natl Acad Sci U S A. 2006 Mar 14;103(11):4128-33. Epub 2006 Mar 6.ÊÊ _x000B__x000B_5. Furne C, Mehlen P. 'The deadly rafts: when lipid rafts regulate dependence receptors.' Med Sci (Paris). 2006 Nov;22(11):909-10._x000B__x000B_6. Nakamura F, Takei K, Goshima Y. 'Signaling mechanism connecting axon guidance molecules and growth cone cytoskeleton.' Tanpakushitsu Kakusan Koso. 2006 May;51(6 Suppl):733-41. _x000B__x000B_7. Chang C, et al. 'MIG-10/lamellipodin and AGE-1/PI3K promote axon guidance and outgrowth in response to slit and netrin.' Curr Biol. 2006 May 9;16(9):854-62. Epub 2006 Apr 20. _x000B_PMID: 16618541_x000B__x000B_8. Xie Y, et al. 'DCC-dependent phospholipase C signaling in netrin-1-induced neurite elongation.' J Biol Chem. 2006 Feb 3;281(5):2605-11. Epub 2005 Nov 30.
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Test
You can block the antibody by the specific target amino acid sequence of peptide.
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Properties
blocking peptide
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Description
Peptides short amino acid chains or epitopes or blocking antagonists. The shortest peptides are dipeptides, consisting of 2 amino acids joined by a single peptide bond, followed by tripeptides, tetra peptides, ... till polypeptides that are long, continuous, and unbranched synthetic peptide chains. These biological oligomers and polymers can be Solid-phase peptide synthesis (SPPS), or in continue produced for custom peptide synthesis projects. The High-efficiency solid phase peptide synthesis (HE-SPPS) is give very low production costs.
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Gene target
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Gene symbol
CLMAT3, SPARC
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Short name
SPARC - Blocking Peptide
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Technique
blocking peptide, Blocking, peptide, blocking peptides, nordc made this blocking amino acid sequence or peptide to block the gene target in a volume of 1. For western blot it is often requested to block your antibody and to see the band of the analyzed protein disappear. This is called a negative control by blocking the WB antibody. peptides
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Isotype
not specified
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Label
unlabeled
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Alternative name
secreted protein, acidic, cysteine-rich (osteonectin) - inhibiting short protein sequence
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Alternative technique
control, peptides
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Alternative to gene target
secreted protein, acidic, cysteine-rich (osteonectin), ON, SPARC and IDBG-54562 and ENSG00000113140 and 6678, extracellular matrix binding, nuclei, Sparc and IDBG-175174 and ENSMUSG00000018593 and 20692, SPARC and IDBG-646341 and ENSBTAG00000014835 and 282077
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