Sheep anti LMW-PTP (HCPTP)

• Catalog number
  P240P
• Price
  Please ask
• Size
  100 μg

• Category
  Primary Antibodies
• Long description
  Low molecular weight protein tyrosine phosphtases, including the human red cell phosphatase, HCPTP, are widely expressed cytosolic proteins of approximately 18kDa that exist in distinct isoforms and are highly selective for phosphotyrosine over phosphoserine or phosphotyrosine. The HCPTP-A and HCPTP-B proteins expressed in human red cells and placenta are fast and slow forms of red cell acid phosphatase5. Like other tyrosine phosphatases, HCPTP is sensitive to inhibition by vanadate, and has a catalytic mechanism that involves formation of a cystinyl-phosphate intermediate6. The crystal structure of the bovine heart derived enzyme BHPTP shows a four-stranded central parallel b sheet with flanking a helices and an active site cysteine residue in the typical tyrosine phosphatase sequence context, CXXXXXR._x000B__x000B_Biological actions and cellular substrates have not been fully elucidated. It is expressed in a wide range of cell types, and structural homologues are expressed in yeast. A catalytically inactive LMW-PTP functions to promote cell division and binds to tyrosine-phosphorylated PDGF receptors1. The HCPTPA isoform interacts with receptor tyrosine kinases, EphB1 and VEGFR2 (flk-1)2,4. Its overexpression inhibits VEGF-induced endothelial proliferation and migration, and its recruitment to EphB1 complexes is crucial to downstream signaling between EphB1 and integrins that mediate cell-matrix attachment3.
• Antibody come from
  The human protein, HCPTPA (GB Accession #M83653) was subcloned into pRSET to express a His6 N terminal fusion protein. This protein was expressed in E. coli and purified to homogeneity on Ni affinity matrix.
• Other description
  Provided as solution in phosphate buffered saline with 0.08% sodium azide. Ammonium Sulfate Precipitation
• Clone
  Polyclonal
• Antigen antibody binding interaction
  Sheep anti LMW-PTP (HCPTP) Antibody
• Antibody is raised in
  Sheep
• Antibody s reacts with
  Human, Mouse, Rat, Bovine
• Antibody s reacts with these species
  This antibody doesn't cross react with other species
• Antibody s specificity
  No Data Available
• Application
  Western Blot, Immunoprecipitation
• Antibody s suited for
  Antbibody can be used for Western blotting and immunoprecipitation (1-10µg/ml. Optimal concentration should be evaluated by serial dilutions.
• Storage
  -20ºC
• Relevant references
  1. Chiarugi, P., Cirri, P., Raugei, G., Camici, G., Dolfi, F., Berti, A., and Ramponi, G. (1995). PDGF receptor as a specific in vivo target for low M(r) protein tyrosine phosphatase. FEBS Letters 372, 49-53._x000B__x000B_2. Huang, L., Sankar, S., Lin, C., Kontos, C.D., Schroff, A.D., Cha, E.H., Feng, S.M., Li, S.F., Yu, Z., Van Etten, R.L., Blanar, M.A., and Peters, K.G. (1999). HCPTPA, a Protein Tyrosine Phosphatase That Regulates Vascular Endothelial Growth Factor Receptor-mediated Signal Transduction and Biological Activity. J.Biol.Chem. 274, 38183-38188._x000B__x000B_3. Huynh-Do, U., Stein, E., Lane, A.A., Liu, H., Cerretti, D.P., and Daniel, T.O. (1999). Surface densities of ephrin-B1 determine EphB1-coupled activation of cell attachment through alphavbeta3 and alpha5beta1 integrins. EMBO J. 18, 2165-2173._x000B__x000B_4. Stein, E., Lane, A.A., Cerretti, D.P., Schoecklmann, H.O., Schroff, A.D., Van Etten, R.L., and Daniel, T.O. (1998). Eph receptors discriminate specific ligand oligomers to determine alternative signaling complexes, attachment, and assembly responses. Genes Dev. 12, 667-678._x000B__x000B_5. Wo, Y.-Y., McCormack, A.L., Shabanowitz, J., Hunt, D.F., Davis, J.P., Mitchell, G.L., and Van Etten, R.L. (1992). Sequencing, cloning, and expression of human red cell-type acid phosphatase, a cytoplasmic phosphotyrosyl protein phosphatase. J Biol Chem 267, 10856-10865._x000B__x000B_6. Zhang, M., Stauffacher, C.V., and VanEtten, R.L. (1995). The three dimensional structure, chemical mechanism and function of the low molecular weight protein tyrosine phosphatases. Adv.Prot.Phosphatases 9, 1-23.
• Protein number
  see ncbi
• Warnings
  This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
• Antibody s link
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

• Gene target
  LMW-PTP   HCPTP  
• Gene symbol
  ACP1, PTPN13, PTPRK, PTPRO, PTPRR, REG1A, PTPN7, PTPN12, PTPN23, PTPRVP
• Short name
  anti LMW-PTP (HCPTP)
• Technique
  anti, antibody to
• Host
  Sheep
• Isotype
  not specified
• Label
  unlabeled
• Species
  Sheep, The sheep (Ovis aries) ELISAs are detecting sheep proteins and antigens. Some epitopes will also recognize goat proteins. Sheep and goats are also used to produce polyclonals. An adult female sheep is referred to as a ewe a male as a ram, a castrated male as a wether, and a younger sheep as a lamb. Sheeps
• Alternative name
  ovine antibody to LMW-PTP (HCPTP)
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:122
• Gene
  ACP1
• Long gene name
  acid phosphatase 1
• Synonyms gene name
  • acid phosphatase 1, soluble
• Synonyms
  • HAAP
  • LMW-PTP
  • LMWPTP
• Synonyms name
  • acid phosphatase of erythrocyte
  • low molecular weight phosphotyrosine protein phosphatase
  • red cell acid phosphatase
• GenBank acession
  • M87546
• Locus
  2p25.3
• Discovery year
  1986-01-01
• Entrez gene record
  52
• Pubmed identfication
  • 457131
  • 1587862
  • 8586411
• RefSeq identity
  • NM_004300
• Classification
  • Acid phosphatases
  • Class II Cys-based phosphatases
• VEGA ID
  OTTHUMG00000086933

Gene info

• Identity
  HGNC:9646
• Gene
  PTPN13
• Long gene name
  protein tyrosine phosphatase non-receptor type 13
• Synonyms gene name
  • protein tyrosine phosphatase, non-receptor type 13 (APO-1/CD95 (Fas)-associated phosphatase)
• Synonyms
  • PTP1E
  • PTP-BAS
  • PTPL1
  • PTP-BL
• Synonyms name
  • APO-1/CD95 (Fas)-associated phosphatase
• Locus
  4q21.3
• Discovery year
  1995-02-17
• Pubmed identfication
  • 8287977
• Classification
  • Protein tyrosine phosphatases non-receptor type
  • PDZ domain containing
  • FERM domain containing
• VEGA ID
  OTTHUMG00000160968

Gene info

• Identity
  HGNC:9674
• Gene
  PTPRK
• Long gene name
  protein tyrosine phosphatase receptor type K
• Synonyms
  • R-PTP-kappa
• GenBank acession
  • L77886
• Locus
  6q22.33
• Discovery year
  1998-02-09
• Entrez gene record
  5796
• Pubmed identfication
  • 9047348
  • 8663237
• Classification
  • Protein tyrosine phosphatases receptor type
  • Fibronectin type III domain containing
  • Immunoglobulin like domain containing
• VEGA ID
  OTTHUMG00000015536

Gene info

• Identity
  HGNC:9678
• Gene
  PTPRO
• Long gene name
  protein tyrosine phosphatase receptor type O
• Synonyms
  • PTPU2
  • GLEPP1
  • PTP-U2
  • PTP-oc
  • NPHS6
• Synonyms name
  • osteoclastic transmembrane protein-tyrosine phosphatase
• GenBank acession
  • U20489
• Locus
  12p12.3
• Discovery year
  1995-02-22
• Entrez gene record
  5800
• Pubmed identfication
  • 7519601
  • 7665166
  • 21722858
• Classification
  • Protein tyrosine phosphatases receptor type
  • Fibronectin type III domain containing
• VEGA ID
  OTTHUMG00000168786

Gene info

• Identity
  HGNC:9680
• Gene
  PTPRR
• Long gene name
  protein tyrosine phosphatase receptor type R
• Synonyms gene
  • PTPRQ
• Synonyms
  • PTPBR7
  • PTP-SL
  • EC-PTP
  • PCPTP1
• GenBank acession
  • D64053
• Locus
  12q15
• Discovery year
  1999-10-28
• Entrez gene record
  5801
• Pubmed identfication
  • 7557444
  • 10393441
• RefSeq identity
  • NM_002849
• Classification
  • Protein tyrosine phosphatases receptor type
• VEGA ID
  OTTHUMG00000169502

Gene info

• Identity
  HGNC:9951
• Gene
  REG1A
• Long gene name
  regenerating family member 1 alpha
• Synonyms gene
  • REG
• Synonyms gene name
  • regenerating islet-derived 1 alpha (pancreatic stone protein, pancreatic thread protein)
• Synonyms
  • PSP
  • PTP
  • PSPS
  • PSPS1
• Synonyms name
  • pancreatic stone protein
  • pancreatic thread protein
• Locus
  2p12
• Discovery year
  1992-06-05
• Entrez gene record
  5967
• Pubmed identfication
  • 2332435
  • 8333731
• RefSeq identity
  • NM_002909
• Classification
  • C-type lectin domain containing
• VEGA ID
  OTTHUMG00000130016

Gene info

• Identity
  HGNC:9659
• Gene
  PTPN7
• Long gene name
  protein tyrosine phosphatase non-receptor type 7
• Synonyms
  • HEPTP
  • LC-PTP
• GenBank acession
  • BC001746
• Locus
  1q32.1
• Discovery year
  1993-03-03
• Entrez gene record
  5778
• Pubmed identfication
  • 1510684
• RefSeq identity
  • NM_002832
• Classification
  • Protein tyrosine phosphatases non-receptor type
• VEGA ID
  OTTHUMG00000035931

Gene info

• Identity
  HGNC:9645
• Gene
  PTPN12
• Long gene name
  protein tyrosine phosphatase non-receptor type 12
• Synonyms
  • PTPG1
  • PTP-PEST
• Locus
  7q11.23
• Discovery year
  1993-03-03
• Pubmed identfication
  • 7509295
• Classification
  • Protein tyrosine phosphatases non-receptor type
• VEGA ID
  OTTHUMG00000023501

Gene info

• Identity
  HGNC:14406
• Gene
  PTPN23
• Long gene name
  protein tyrosine phosphatase non-receptor type 23
• Synonyms
  • DKFZP564F0923
  • KIAA1471
  • HD-PTP
• GenBank acession
  • AB025194
• Locus
  3p21.31
• Discovery year
  2001-02-15
• Entrez gene record
  25930
• Pubmed identfication
  • 11095967
• RefSeq identity
  • NM_015466
• Classification
  • Protein tyrosine phosphatases non-receptor type
• VEGA ID
  OTTHUMG00000133520

Gene info

• Identity
  HGNC:13421
• Gene
  PTPRVP
• Long gene name
  protein tyrosine phosphatase receptor type V, pseudogene
• Synonyms gene
  • PTPRV
• Synonyms
  • OST-PTP
  • ESP
• GenBank acession
  • AJ629456
• Locus
  1q32.1
• Discovery year
  2005-06-07
• Entrez gene record
  148713
• Pubmed identfication
  • 15358244
• RefSeq identity
  • XM_086287
• Classification
  • Protein tyrosine phosphatases receptor type
• VEGA ID
  OTTHUMG00000040524

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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