Sheep anti E1A (adenovirus early region 1)

• Catalog number
  A100P
• Price
  Please ask
• Size
  100 μg

• Category
  Primary Antibodies
• Long description
  The immunogen for the anti-EIA was a recombinant E1A protein recognizing a 43kD protein. EIA gene encodes potent onco-proteins that modify the normal transcriptional growth regulation of key cellular genes and thus alter cell cycle control. E1A protein has both transforming and trans-activating activities. Plays a role in viral genome replication by driving entry of quiescent cells into the cell cycle. Disrupts the function of host retinoblastoma protein RB1/pRb and isoform early E1A 26 kDa protein stabilizes TP53, which are key regulators of the cell cycle. Induces the disassembly of the E2F1 transcription factors from RB1 by direct competition for the same binding site on RB1, with subsequent transcriptional activation of E2F1-regulated S-phase genes. Inactivation of the ability of RB1 to arrest the cell cycle is critical for cellular transformation, uncontrolled cellular growth and proliferation induced by viral infection. Stimulation of progression from G1 to S phase allows the virus to efficiently use the cellular DNA replicating machinery to achieve viral genome replication. Interaction with RBX1 and CUL1 inhibits ubiquitination of the proteins targeted by SCF(FBW7) ubiquitin ligase complex, and may be linked to unregulated host cell proliferation. The tumorigenesis-restraining activity of E1A may be related to the disruption of the host CtBP-CtIP complex through the CtBP binding motif
• Antibody come from
  Recombinant E1A protein recognizing a 43kD protein
• Other description
  Provided as solution in phosphate buffered saline with 0.08% sodium azide. Ammonium Sulfate Precipitation
• Clone
  Polyclonal
• Antigen antibody binding interaction
  Sheep anti E1A (adenovirus early region 1) Antibody
• Antibody is raised in
  Sheep
• Antibody s reacts with
  Human, Rat
• Antibody s reacts with these species
  This antibody doesn't cross react with other species
• Antibody s specificity
  No Data Available
• Application
  Immunoprecipitation
• Antibody s suited for
  Western Blot suggested dilution 1- 10 ug /ml
• Storage
  -20ºC
• Relevant references
  1. Chatton, B., et al. 'Transcriptional activation by the adenovirus larger E1a product is mediated by members of the cellular transcription factor ATF family which can directly associate with E1a.' Mol. Cell. Biol., 13, 561-570 (1993)
• Protein number
  see ncbi
• Warnings
  This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
• Antibody s link
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

• Gene target
  E1A   adenovirus   early   region  
• Gene symbol
  ETV4
• Short name
  anti E1A (adenovirus early region 1)
• Technique
  anti, antibody to
• Host
  Sheep
• Isotype
  not specified
• Label
  unlabeled
• Species
  Sheep, The sheep (Ovis aries) ELISAs are detecting sheep proteins and antigens. Some epitopes will also recognize goat proteins. Sheep and goats are also used to produce polyclonals. An adult female sheep is referred to as a ewe a male as a ram, a castrated male as a wether, and a younger sheep as a lamb. Sheeps
• Alternative name
  ovine antibody to E1A (adenovirus early region 1)
• Alternative technique
  antibodies
• Tissue
  region
• Virus
  adenovirus

Gene info

• Identity
  HGNC:3493
• Gene
  ETV4
• Long gene name
  ETS variant transcription factor 4
• Synonyms gene name
  • ets variant gene 4 (E1A enhancer-binding protein, E1AF)
  • ETS variant 4
• Synonyms
  • E1A-F
  • E1AF
  • PEA3
• Synonyms name
  • E1A enhancer binding protein
• GenBank acession
  • U18018
• Locus
  17q21.31
• Discovery year
  1995-03-27
• Entrez gene record
  2118
• Pubmed identfication
  • 8530053
  • 1547944
• RefSeq identity
  • NM_001986
• Classification
  • ETS transcription factor family
• VEGA ID
  OTTHUMG00000180884

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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