Sheep anti chk 1 kinase

• Catalog number
  K100P
• Price
  Please ask
• Size
  250 μg

• Category
  Primary Antibodies
• Long description
  The immunogen for anti-human chk-1 was a protein corresponding to amino acid sequence 1-474 and having a molecular weight of 54 kD. One mechanism by which checkpoints maintain the fidelity of cell cycle events is through blocking mitosis in response to unreplicated or damaged DNA. In most species this occurs through inhibiting activation of the cyclic-dependent kinase cdc2, which regulates entry in to mitosis. Chk1, a kinase involved in the DNA damage checkpoint response can phosphorylate Cdc25C an activator of cdc2. It is hypothesized that chk 1 induces serine 216 phosphorylation of Cdc 25C and subsequent binding negatively regulated Cdc25C, thus preventing it from activating cdc2.
• Antibody come from
  A protein corresponding to amino acid sequence 1-474 and having a molecular weight of 54 kD
• Other description
  Provided as solution in phosphate buffered saline with 0.08% sodium azide. Ammonium Sulfate Precipitation
• Clone
  Polyclonal
• Antigen antibody binding interaction
  Sheep anti chk 1 kinase Antibody
• Antibody is raised in
  Sheep
• Antibody s reacts with
  Human, Mouse, Rat, Monkey
• Antibody s reacts with these species
  This antibody doesn't cross react with other species
• Antibody s specificity
  No Data Available
• Application
  Western Blot, Immunoprecipitation
• Antibody s suited for
  Applications: Western Blot at 1 Ð 10 ug/ml.
• Storage
  -20ºC
• Relevant references
  1. Wang, Y., et al. 'CHK1 kinase activity assay.' Methods Mol Biol 2004, 281, 143-151._x000B__x000B_2. Yarden, R.I., et al. 'BRCA1 regulates the G2/M checkpoint by activating Chk1 kinase upon DNA damage.' Nat Genet 2002, 30, 285-289_x000B__x000B_3. Nakajo, N., et al. 'Involvement of Chk1 kinase in prophase I arrest of Xenopus oocytes.' Dev Biol 1999, 207, 432-444
• Protein number
  see ncbi
• Warnings
  This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
• Antibody s link
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

• Gene target
  chk   kinase  
• Short name
  anti chk 1 kinase
• Technique
  anti, antibody to
• Host
  Sheep
• Isotype
  not specified
• Label
  unlabeled
• Species
  Sheep, The sheep (Ovis aries) ELISAs are detecting sheep proteins and antigens. Some epitopes will also recognize goat proteins. Sheep and goats are also used to produce polyclonals. An adult female sheep is referred to as a ewe a male as a ram, a castrated male as a wether, and a younger sheep as a lamb. Sheeps
• Alternative name
  ovine antibody to chk 1 phosphorylation catalyst
• Alternative technique
  antibodies

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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