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Category
Primary Antibodies
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Long description
bcl-x is a bcl-2-related gene that can function as a regulator of programmed cell death (apoptosis) independent of bcl-2. Alternative splicing results in two distinct bcl-x mRNAs. The larger mRNA gives rise to a protein product, bcl-xl, which is similar in size and predicted structure to bcl-2 (1). The smaller mRNA gives rise to bcl-xS. bcl-x immunoreactivity has been detected in a wide variety of cell types and the protein is typically present in the cytosol in association with the mitochondrial periphery, a property shared with bcl-2 however membrane bound forms of bcl-x have been demonstrated in thymocytes (2-4). Following the induction of apoptosis all of the bcl-x protein shifts to the membrane form (2). Of the two isoforms of bcl-x, the long (bcl-xl) is the most abundant mRNA species expressed in embryonic and adult tissues and most likely differs from bcl-2 in its regulatory activity on cell differentiation through controlled tissue specific expression (1,3). Like its homolog bcl-2, bcl-x undergoes phosphorylation, a modification that requires that a specific 60 amino acid loop region be intact, which in turn appears to regulate activity (5,6). Structurally, based on 3D-structure analysis, bcl-x forms pH sensitive cation-selective ion channels in membranes a property shared with the pore forming domains of several bacterial toxins (7). Bcl-xl has been shown to modify the cellÕs response to oxidants, to participate in resistance to chemotherapeutic agents and radiation, and to play a key role in the development of the developing CNS (8-10).
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Antibody come from
A synthetic peptide corresponding to amino acids 3 to 14 of the human bcl-xl sequence
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Other description
Provided as solution in phosphate buffered saline with 0.08% sodium azide. Ammonium Sulfate Precipitation
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Clone
Polyclonal
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Antigen antibody binding interaction
Sheep anti bcl -xl Antibody
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Antibody is raised in
Sheep
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Antibody s reacts with
Human
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Application
Western Blot
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Antibody s suited for
Application: Western Blot at 1-5 ug/ml. Positive control MCF-7 cells.
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Storage
-20ºC
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Relevant references
1. Boise L.H., et al. (1993) Cell 74: 597-608. _x000B__x000B_2. Krajewski S., et al. (1994) Cancer Res 54:5501-5507. _x000B__x000B_3. Gonzalez-Garcia M., et al. (1994) Development 120: 3033-3042. _x000B__x000B_4. Hsu, Y.T., et al. (1997) Proc. Natl. Acad. Sci USA 94: 3668-3672. _x000B__x000B_5. Benito, A., et al. (1997) Leukemia 11 940-944. _x000B__x000B_6. Chang, B.S., et al. (1997) EMBO J. 16: 968-977. _x000B__x000B_7. Minn, A.J., et al. (1997) Nature 385: 353-357. _x000B__x000B_8. Fang W., et al. (1995) J Immunol 155: 66-75. _x000B__x000B_9. Datta R., et al. (1995) Cell Growth Differ 6:363-370. _x000B__x000B_10. Shindler, K.S., et al. (1997) J. Neurosci. 17:3112-3119.
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
