Rat anti Laminin

• Catalog number
  MUB1100P
• Price
  Please ask
• Size
  0,1 mg

• Category
  Primary Antibodies
• Long description
  Basement membrane components play an important role in cell adhesion, locomotion, differentiation and embryonic compartmentalization. Laminin anchors cells to the basement membrane through interactions with integrins at the cell surface and collagens in the basement membrane.
• Antibody come from
  A5 is a Rat monoclonal IgG2a antibody derived by fusion of Mouse myeloma cells with spleen cells from a Fisher Rat immunized with a laminin preparation from the EHS Mouse tumor.
• Other description
  Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
• Clone
  A5
• Antigen antibody binding interaction
  Rat anti Laminin Antibody
• Antibody is raised in
  Rat
• Antibody s reacts with
  Human,Mouse,Swine
• Antibody s reacts with these species
  This antibody doesn't cross react with other species
• Antibody s specificity
  A5 reacts with laminin.
• Research interest
  Cytoskeleton, Neurobiology
• Application
  Immunocytochemistry,Immunohistochemistry (frozen), Immunofluorescence
• Antibody s suited for
  A5 is suitable for immunocytochemistry, immunofluorescence and immunohistochemistry on frozen tissues. Optimal antibody dilution should be determined by titration; recommended range is 1:25 – 1:200 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent.
• Storage
  Store at 4°C, or in small aliquots at -20°C.
• Relevant references
  Ljubimov, A. V., Bartek, J., Couchman, J. R., Kapuller, L. L., Veselov, V. V., Kovarik, J., Perevoshchikov, A. G., and Krutovskikh, V. A. (1992). Distribution of individual components of basement membrane in Human colon polyps and adenocarcinomas as revealed by monoclonal antibodies. Int J Cancer 50, 562-66. Ljubimova, J.Y., Lakhter, A.J., Loksh, A., Yong, W.H., Riedinger, M.S., Miner, J.H., Sorokin, L.M., Ljubimov, A.V., Black, K.L. (2001). Overexpression of alpha4, chain-containing laminins in Human glial tumors identified by gene microarray analysis. Cancer Research 61, 5601-10.
• Protein number
  see ncbi
• Warnings
  This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
• Antibody s link
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
• About
  Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
• Latin name
  Rattus norvegicus

• Gene target
  Laminin  
• Gene symbol
  LAMB2, LAMA5, LAMA4
• Short name
  anti Laminin
• Technique
  anti, antibody to
• Host
  Rat
• Isotype
  IgG2a
• Label
  unlabeled
• Species
  Rat, Rats
• Alternative name
  Rat antibody to Laminin
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:6487
• Gene
  LAMB2
• Long gene name
  laminin subunit beta 2
• Synonyms gene
  • LAMS
• Synonyms gene name
  • laminin, beta 2 (laminin S)
• Synonyms
  • NPHS5
• Synonyms name
  • laminin S
• Locus
  3p21.31
• Discovery year
  1992-05-06
• Entrez gene record
  3913
• Pubmed identfication
  • 2922051
  • 10393422
• RefSeq identity
  • NM_002292
• Classification
  • Laminin subunits
• VEGA ID
  OTTHUMG00000156807

Gene info

• Identity
  HGNC:6485
• Gene
  LAMA5
• Long gene name
  laminin subunit alpha 5
• Synonyms gene name
  • laminin, alpha 5
• GenBank acession
  • AF443072
• Locus
  20q13.33
• Discovery year
  1997-10-30
• Entrez gene record
  3911
• Pubmed identfication
  • 9271224
• RefSeq identity
  • NM_005560
• Classification
  • Laminin subunits
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000032908

Gene info

• Identity
  HGNC:6484
• Gene
  LAMA4
• Long gene name
  laminin subunit alpha 4
• Synonyms gene name
  • laminin, alpha 4
• Synonyms
  • LAMA3
• Locus
  6q21
• Discovery year
  1993-07-26
• Entrez gene record
  3910
• Pubmed identfication
  • 7959779
• RefSeq identity
  • NM_001105206
• Classification
  • Laminin subunits
• VEGA ID
  OTTHUMG00000015386
• Locus Specific Databases
  • LRG_433

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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