-
Category
Primary Antibodies
-
Long description
Interleukin 5 is a T cell-derived glycoprotein which stimulates eosinophil colony formation and is an eosinophil differentiation factor in humans and mice. It is also a growth and differentiation factor for mouse but not human B cells.
-
Antibody come from
Recombinant human p38 MAPK
-
Other description
Provided as solution in phosphate buffered saline, pH 7.3, with 1.0 mg/ml BSA and 0.1% sodium azide. Protein A/G Chromatography
-
Clone
TRFK-4
-
Antigen antibody binding interaction
Rat anti Interleukin-5 antibody conjugated to Biotin Antibody
-
Antibody is raised in
Rat
-
Antibody s reacts with
Mouse
-
Antibody s reacts with these species
This antibody doesn't cross react with other species
-
Antibody s specificity
No Data Available
-
Application
ELISA as a detection antibody
-
Antibody s suited for
Optimal concentration should be evaluated by serial dilutions. For Western blotting, the recommended concentration is 1 µg/mL
-
Storage
-20ºC
-
Relevant references
1. Rouse, J., P. Cohen, S. Trigon, M. Morange, A. Alonso-Llamazares, D. Zamanillo, T. Hunt, and A.R. Nebreda (1994) A novel kinase cascade triggered by stress and heat shock that stimulates MAPKAP kinase-2 and phosphorylation of the small heat shock proteins. Cell 78(6):1027-1037._x000B_2. Han, J., J.D. Lee, L. Bibbs, and R.J. Ulevitch (1994) A MAP kinase targeted by endotoxin and hyperosmolarity in mammalian cells. Science 265(5173):808-811. _x000B_3. Karasewski, L. and A. Ferreira (2003) MAPK signal transduction pathway mediates agrin effects on neurite elongation in cultured hippocampal neurons. J. Neurobiol. 55(1):14-24._x000B_4. He, R., et al. (2003) Serum amyloid A induces IL-8 secretion through a G protein-coupled receptor, FPRL1/LXA4R. Blood 101(4):1572-1581._x000B_5. Janulis, M., et al. (2001) A novel mitogen-activated protein kinase is responsive to Raf and mediates growth factor specificity. Mol. Cell. Biol. 21(6):2235-2247._x000B_6. Nanki, T., et al. (2001) Chemokines regulate IL-6 and IL-8 production by fibroblast-like synoviocytes from patients with rheumatoid arthritis. J. Immunol. 167(9):5381-5385._x000B_7. Tong, T., et al. (2001) Involvement of the MAP kinase pathways in induction of GADD45 following UV radiation. Exp. Cell Res. 269(1):64-72._x000B_
-
Protein number
see ncbi
-
Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
-
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
-
Properties
If you buy Antibodies supplied by nordc they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
-
Conjugation
Biotinylated
-
About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
-
Latin name
Rattus norvegicus
-
French translation
anticorps
