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Category
Primary Antibodies
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Long description
Basement membrane components play an important role in cell adhesion, locomotion, differentiation and embryonic compartmentalization. Although the precise composition and structure of basal laminae varies from tissue to tissue, as well as within the same tissue at different developmental stages, most mature basal laminae contain type IV collagen, laminins, heparan sulphate proteoglycans and entactin (or niDogen). Entactin is a sulphated glycoprotein of approximately 150 kD.
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Antibody come from
ELM1 is a Rat monoclonal IgG2a antibody derived by fusion of X63 Ag 8.653 Mouse myeloma cells with spleen cells from a Fisher Rat immunized with a partially purified preparation of laminin from the EHS Mouse tumor.
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Other description
Each vial contains 500 µl 0.2 mg/ml purified monoclonal antibody in phosphated buffered saline (PBS) containing 50% glycerol + 0.2% bovine serum albumin (BSA) + 0.02% sodium azide.
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Clone
ELM1
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Antigen antibody binding interaction
Rat anti Entactin / NiDogen Antibody
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Antibody is raised in
Rat
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Antibody s reacts with
Mouse
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
ELM1 reacts with entactin.
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Research interest
Cell adhesion
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Application
Immunocytochemistry,Immunohistochemistry (frozen),Western blotting
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Antibody s suited for
ELM1 is suitable for immunoblotting, immunocytochemistry and immunohistochemistry on frozen tissues. Optimal antibody dilution should be determined by titration; recommended range is 1:25 – 1:200 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 – 1:1000 for immunoblotting applications.
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Storage
Store at 4°C, or in small aliquots at -20°C.
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Relevant references
Ljubimov, A. V., Afanasjeva, A. V., Litvinova, L. V., Senin, V.M. (1986). Basement membrane components produced by a Mouse ascites teRatocarcinoma TB24, Exp Cell Res 165, 530-40. Couchman, J. R., and Ljubimov, A. V. (1989). Mammalian tissue distribution of a large heparan sulfate proteoglycan detected by monoclonal antibodies. Matrix 9, 311-321.
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Protein number
P10493
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Latin name
Rattus norvegicus
