Rap 2A Recombinant Protein

• Catalog number
  X1213
• Price
  Please ask
• Size
  25 μg

• Category
  Proteins & Peptides
• Long description
  Rap2B is a 21 kDa small GTP-binding protein that that exhibits 46% identity with Ras proteins and is similarly post-translationally modified by farnesyl and palmitate groups. It colocalizes with several markers of the Golgi complex, thus indicating that its site of function was distinct from that of Rap1A. In contrast to Rap1A, Rap2B protein does not interfere with Ras-induced transformation suggesting that the activity of Rap proteins goes beyond the regulation of Ras protein function. Ras proteins provide a crucial link in the intracellular signalling cascades. Ras proteins are members of the small G protein family. These proteins are characterized by a molecular weight between 20 and 30 kDa, the ability to bind GTP or GDP and a low intrinsic GTPase activity. These proteins relay signals from the plasma membrane activated by receptor tyrosine kinases to the nucleus via a network of serine/threonine kinases. Ras proteins belong to a family of approximately 40 mammalian and 60 non-mammalian proteins. Ras proteins were first discovered as the hyperactive products of mutant Ras genes which promote cancer by disrupting the normal controls on cell proliferation and differentiation; about 30% of human cancers have mutations in a Ras gene.
• Antibody come from
  Recombinant full length human Rap1A protein expressed in e.coli
• Other description
  Provided in 50mM Tris (pH 7.5) solution containing 150 mM NaCl, 10 mM MgCl2, 1 mM DTT, 10% glycerol .
• Clone
  N/A
• Antigen antibody binding interaction
  Rap 2A Recombinant Protein
• Antibody is raised in
  N/A
• Antibody s reacts with
  Human
• Antibody s reacts with these species
  This antibody doesn't cross react with other species
• Antibody s specificity
  No Data Available
• Application
  Affinity Binding
• Antibody s suited for
  Rap2B protein is provided as a functional G-protein and can be used as a Western blot control for antibodies to Rap2B protein or in assays to study to protein-protein interactions of Rap2B and related proteins with regulatory proteins. Optimal concentration should be evaluated by serial dilutions.
• Storage
  -80ºC
• Relevant references
  1. Pizon, V., et al. 'Association of Rap1a and Rap1b proteins with late endocytic/phagocytic compartments and Rap2a with the Golgi complex.' J. Cell. Sci. 1994, 107, 1661-1670 _x000B_2. Pasheva, E., et al. 'Characterization of the Ras-related RAP2A protein expressed in the baculovirus-insect cell system: processing of the protein in insect cells and comparison with the bacterially produced unprocessed form.' Biochem. Biophys. Res. Commun. 1994, 198, 973-982 _x000B__x000B_3. Ohba, Y., et al. 'Rap2 as a slowly responding molecular switch in the Rap1 signaling cascade.' Mol. Cell Biol. 2000, 20, 6074-6083
• Protein number
  see ncbi
• Warnings
  This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
• Antibody s link
• Source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Rap   Protein  
• Short name
  Rap 2A Recombinant Protein
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. nordc advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Isotype
  N/A
• Label
  unlabeled
• Alternative name
  Rap 2A Rec. Protein
• Alternative technique
  rec

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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