Rabbit IgG antibody conjugated with FITC

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• Catalog number
  RIgG/FITC
• Price:

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  Ask for price
• Size
  1 mL

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• Category
  Isotype control
• Long description
  As an efficient general counterstain for cell and tissue substrates with immunoconjugates with a label contrasting with the greenish-yellow fluorescence of this product. The purpose of counterstaining is to enhance the optical contrast of the immunospecific staining against a counterstained background. Working dilutions fare usually between 1:20 an 1:80.
• Antibody come from
  n/a
• Other description
  Fluorochrome coupled purified rabbit IgG in phosphate buffered saline (pH7.2). No preservative added
• Clone
  see datasheet
• Antigen antibody binding interaction
  Rabbit IgG antibody conjugated with FITC Isotype control
• Antibody is raised in
  see datasheet
• Antibody s reacts with
  Rabbit
• Antibody s reacts with these species
  This antibody doesn't cross react with other species
• Antibody s specificity
  Fluorescein isothiocyanate-conjugated polyclonal Rabbit IgG.
• Research interest
  Veterinary
• Application
  ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence
• Antibody s suited for
  ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence. The conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at -20°C. Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water. Spin down to remove insoluble particles, divide into small aliquots, and store frozen at or below -20°C. Prior to use an aliquot is thawed at ambient temperature, spun down again, and used to prepare working dilutions by adding sterile PBS pH7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the conjugate. Diluted product should be stored at +4°C, not refrozen, and preferably used the same day.
• Storage
  Lyophilized at +4°C--at least 10 years. Reconstituted at or below -20°C--3-5 years. Reconstituted at +4°C--7 days.
• Relevant references
  no information yet
• Protein number
  see ncbi
• Warnings
  This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
• Antibody s link
• Properties
  If you buy Antibodies supplied by nordc they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This nordc Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. nordc FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody
• About
  Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Rabbits are used for polyclonal antibody production by nordc. Rabbit antibodies are very stable and can be stored for several days at room temperature. nordc adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
• Latin name
  Oryctolagus cuniculus
• French translation
  anticorps

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• Gene target
  conjugated   with  
• Short name
  Rabbit IgG antibody conjugated with FITC
• Technique
  Antibody, Rabbit, FITC, IgG, antibodies against human proteins, antibodies for, antibody Conjugates, Fluorescein, IgGs
• Host
  Rabbit, Rabbits
• Isotype
  see datasheet, IgG
• Label
  FITC
• Alternative name
  production species: rabbit Immunoglobulin G (antibody to-) coupled including fluorecein
• Alternative technique
  antibodies, rabbit-anti, fluorescine, immunoglobulins

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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