Rabbit anti VEGF-A (Vascular Endothelial Growth Factor A) antibody conjugated to Biotin
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Catalog numberX2003P
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PricePlease ask
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Size100 μg
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CategoryPrimary Antibodies
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Long descriptionVEGF (Vascular Endothelial Growth Factor) is a homodimeric, disulfide-linked glycoprotein involved in angiogenesis which promotes tumor progression and metastasis. It exhibits potent mitogenic and permeability inducing properties specific for the vascular endothelium. Of the four isoforms of VEGF, the smaller two, VEGF 165 and VEGF 121, are secreted proteins and act as diffusible agents, whereas the larger two (VEGF 189 and VEGF 206) remain cell associated.
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Antibody come fromThe antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human c-Kit that contains tyrosine 721. (Mouse, Rat have 82% homology and are expected to work but have not been tested)
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Other descriptionProvided as solution in phosphate buffered saline, pH 7.3, with 1.0 mg/ml BSA, 50% glycerol and 0.05% sodium azide. Protein A/G Chromatography
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ClonePolyclonal
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Antigen antibody binding interactionRabbit anti VEGF-A (Vascular Endothelial Growth Factor A) antibody conjugated to Biotin Antibody
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Antibody is raised inRabbit
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Antibody s reacts withHuman
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Antibody s reacts with these speciesThis antibody doesn't cross react with other species
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Antibody s specificityNo Data Available
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ApplicationELISA (As a detection antibody)
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Antibody s suited forOptimal concentration should be evaluated by serial dilutions. For Western blotting applications, we recommend using the antibody at a 1:1000 dilution
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Storage-20ºC
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Relevant references1. Jin, X., et al. (2005) Anti-apoptotic action of stem cell factor on oocytes in primordial follicles and its signal transduction. Mol. Reprod. Dev. 70(1):82-90._x000B_2. Koma, Y., et al. (2005) Distinct role for c-kit receptor tyrosine kinase and SgIGSF adhesion molecule in attachment of mast cells to fibroblasts. Lab Invest. 85(3):426-435._x000B_3. Lennartsson, J., R. Shivakrupa and D. Linnekin (2004) Synergistic growth of stem cell factor and granulocyte macrophage colony-stimulating factor involves kinaseÐdependent and Ðindependent contributions from c-Kit. J. Biol. Chem. 279(43):44544-44553._x000B_4. Hashimoto, K., et al. (2003) Necessity of tyrosine 719 and phosphatidylinositol 3Õ-kinase-mediated signal pathway in constitutive activation and oncogenic potential of c-kit receptor tyrosine kinase with the Asp814Val mutation. Blood 101(3):1094-1102. _x000B_5. Mol, C.D. et al. (2003) Structure of a c-kit product complex reveals the basis for kinase tansactivation. J. Biol. Chem. 278(34):31461-31464._x000B_6. Trieselmann, N.Z., et al. (2003) Mast cells stimulated by membrane-bound, but not soluble, steel factor are dependent on phospholipase C activation. Cell Mol. Life Sci. 60(4):759-766. _x000B_7. Liang, X., et al. (2002) Phosphatidylinositol 3-kinase and Src family kinases are required for phosphorylation and membrane recruitment of Dok-1 in c-Kit signaling. J. Biol. Chem. 277(16):13732-13738._x000B_8. Gommerman, J.L., et al. (2000) Differential stimulation of c-Kit mutants by membrane-bound and soluble Steel Factor correlates with leukemic potential. Blood 96(12):3734-3742._x000B_9. Taylor, M.L., and D.D. Metcalfe (2000) Kit signal transduction. Hematol. Oncol. Clin. North Am. 14(3):517-535._x000B_10. Timokhina, I., et al. (1998) Kit signaling through PI 3-kinase and Src kinase pathways: an essential role for Rac1 and JNK activation in mast cell proliferation. EMBO J. 17(21):6250-6262.
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Protein numbersee ncbi
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WarningsThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.
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PropertiesIf you buy Antibodies supplied by nordc they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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ConjugationBiotinylated
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AboutRabbits are used for polyclonal antibody production by nordc. Rabbit antibodies are very stable and can be stored for several days at room temperature. nordc adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin nameOryctolagus cuniculus
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French translationanticorps
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Gene target
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Gene symbolVEGFA, VEGFD, VEGFC, HLA-A, IGHV5-10-1, CIDEA, RPA1, CDC37P1, OR1Q1
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Short nameRabbit anti VEGF-A (Vascular Endothelial Growth Factor A) antibody conjugated
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TechniqueAntibody, Rabbit, anti, antibody to, antibodies against human proteins, antibodies for, antibody Conjugates
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HostRabbit, Rabbits
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Isotypenot specified
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LabelBiotin
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Alternative nameproduction species: rabbit antibody to VEGF-A (Vascular Endothelial Growth Factor A) (antibody to-) coupled to biotinilated
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Alternative techniqueantibodies, rabbit-anti
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Tissuevascular
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Gene info
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Identity
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Gene
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Long gene namevascular endothelial growth factor A
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Synonyms gene
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Synonyms gene name
- vascular endothelial growth factor
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Synonyms
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GenBank acession
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Locus
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Discovery year1994-01-10
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- VEGF family
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namevascular endothelial growth factor D
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Synonyms gene
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Synonyms gene name
- c-fos induced growth factor (vascular endothelial growth factor D)
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Synonyms
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GenBank acession
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Locus
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Discovery year1997-03-27
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- VEGF family
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namevascular endothelial growth factor C
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1996-10-26
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- VEGF family
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemajor histocompatibility complex, class I, A
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GenBank acession
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Locus
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Discovery year2001-06-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- C1-set domain containing
- Histocompatibility complex
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 5-10-1
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Synonyms gene
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Synonyms gene name
- immunoglobulin heavy variable 5-A (provisional, gene/pseudogene)
- immunoglobulin heavy variable 5-A (provisional)
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-04
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namecell death inducing DFFA like effector a
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Synonyms gene name
- cell death-inducing DFFA-like effector a
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-07-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namereplication protein A1
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Synonyms gene name
- replication protein A1 (70kD)
- replication protein A1, 70kDa
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Synonyms
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GenBank acession
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Locus
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Discovery year1993-12-14
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Nucleotide excision repair
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namecell division cycle 37 pseudogene 1
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Synonyms gene name
- cell division cycle 37 homolog (S. cerevisiae) pseudogene 1
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Synonyms
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Locus
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Discovery year2009-01-29
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameolfactory receptor family 1 subfamily Q member 1
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Synonyms gene
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Synonyms gene name
- olfactory receptor, family 1, subfamily Q, member 1
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Synonyms
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Locus
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Discovery year1999-12-09
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Entrez gene record
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Classification
- Olfactory receptors, family 1
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data