-
Category
Primary Antibodies
-
Long description
Upon cell activation Presqualene diphosphate (PSDP)2, a bioactive lipid, rapidly remodels to presqualene monophosphate (PSMP). A newly identified and characterized phosphatase (phosphatidic acid phosphatase domain containing 2 (PPAPDC2)) has recent;y been shown to convert PSDP to PSMP. Also known as Phosphatidic acid phosphatase type 2 domain-containing protein 2. Unlike the related polyisoprenyl phosphate farnesyl diphosphate (FDP), PSDP has been shown not to be a substrate for type 2 lipid phosphate phosphohydrolases. PSDP phosphatase activity has been identified in activated human neutrophil (PMN) extracts. Recombinant PPAPDC2 displays diphosphate phosphatase activity with substrate preference for PSDP > FDP phosphatidic acid. PPAPDC2 activity is independent of Mg2+ and optimal at pH 7.0-8.0. PPAPDC2 mRNA has been detected in human PMN, and is widely expressed in human tissues. PPAPDC2 expressed in human PMN is first lipid phosphate phosphohydrolase identified for PSDP. The regulation of this enzymeÕs activity has important implications for PMN activation in innate immunity.
-
Antibody come from
Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with cytokeratin preparation extracted from human esophagus and mouse SP2/0 myeloma cells.
-
Other description
Provided as solution in phosphate buffered saline with 0.08% sodium azide.
-
Clone
Polyclonal
-
Antigen antibody binding interaction
Rabbit anti Phosphatidic Acid Phosphatase Domain Containing 2 (PPAPDC2) Antibody
-
Antibody is raised in
Rabbit
-
Antibody s reacts with
Human
-
Antibody s reacts with these species
This antibody doesn't cross react with other species
-
Antibody s specificity
No Data Available
-
Application
Western Blot, Immunoprecipitation
-
Antibody s suited for
Antibody can be used for immunoblotting (1:100-1:1000) and immunohistochemistry on frozen tissue sections (1:25-1:200). Optimal concentration should be evaluated by serial dilutions.
-
Storage
-80ºC
-
Relevant references
1. van Muijen, G. N., et al. (1986). Cell type heterogeneity of cytokeratin expression in complex epithelia and carcinomas as demonstrated by monoclonal antibodies specific for cytokeratins nos. 4 and 13, Exp Cell Res 162, 97-113._x000B_2. Weikel, W., et al. (1987). Characterization of subcolumnar reserve cells and other epithelia of human uterine cervix. Demonstration of diverse cytokeratin polypeptides in_x000B_reserve cells, Virchows Arch B Cell Pathol Incl Mol Pathol 54, 98-110._x000B_3. Smedts, F., et al. (1990). Changing patterns of keratin expression during progression of cervical intraepithelial neoplasia, Am J Pathol 136, 657-68._x000B_4. van Niekerk, C. C., et al. (1991). Marker profile of different phases in the transition of normal human ovarian epithelium to ovarian carcinomas, Am J Pathol 138, 455-63._x000B_5. Smedts, F., et al. (1992). Keratin expression in cervical cancer, Am J Pathol 141, 497-511. _x000B_6. Bauwens, L. J., et al. (1992). Expression of intermediate filament proteins in the adult human vestibular labyrinth, Ann Otol Rhinol Laryngol 101, 479-86._x000B_7. Van Niekerk, C. C., et al. (1993). Changes in expression of differentiation markers between normal ovarian cells and derived tumors, Am J Pathol 142, 157-77._x000B_8. van Dorst, E. B., et al. (1998). The limited difference between keratin patterns of squamous cell carcinomas and adenocarcinomas is explicable by both cell lineage and state of differentiation of tumour cells, J Clin Pathol 51, 679-84.
-
Protein number
see ncbi
-
Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
-
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
-
About
Rabbits are used for polyclonal antibody production by nordc. Rabbit antibodies are very stable and can be stored for several days at room temperature. nordc adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
-
Latin name
Oryctolagus cuniculus
