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Category
Primary Antibodies
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Long description
The p73 exhibits sequence homology to the p53's transcriptional activation, DNA binding, and oligomerization domains. However, adenovirus E1B 55K, simian virus 40 T, and human papillomavirus E6 do not physically interact with p73 indicating that the functions of these two proteins may differ under physiological conditions. p73 is expressed in either a full-length form ( ) (MW ~80kDa) or a shorter ( ) (MW ~70kDa) mRNA variant, with exon 13 spliced out._x000B_
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Antibody come from
Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with purified rat SNAP25 and mouse NS1 cells.
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Other description
Provided as solution in phosphate buffered saline with 0.08% sodium azide. Ammonium Sulfate Precipitation
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Clone
Polyclonal
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Antigen antibody binding interaction
Rabbit anti p73 (C Terminal) Antibody
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Antibody is raised in
Rabbit
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Antibody s reacts with
Human, Mouse
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Application
Western Blot, Immunoprecipitation
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Antibody s suited for
Antibody can be used for Western blotting (1-5 µg/ml) and immunohistochemistry on paraformaldehyde fixed CNS tissues (1-5 µg/ml). Does not stain reduced proteins. Optimal concentration should be evaluated by serial dilutions.
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Storage
-20ºC
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Relevant references
1. Ritchie, T.C., et al. 'A nerve terminal protein with a selective distribution in spinal cord and brain.' J. Neurosci. 1989: 9, 2697-2709_x000B__x000B_2. Zhao, N., et al. 'Cloning and sequence analysis of the human SNAP25 cDNA.' Gene 1994: 145, 313-314_x000B__x000B_3. McMahon , H.T., et al. 'Synaptic core complex of synaptobrevin, syntaxin and SNAP25 forms high affinity alpha-SNAP binding site.' J. Biol. Chem. 1995: 270, 2213-2217_x000B__x000B_4. Gerona, R.R., et al. 'The C terminus of SNAP25 is essential for Ca(2+)-dependent binding of synaptotagmin to SNARE complexes.' J. Biol. Chem. 2000: 275, 6328-6336
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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About
Rabbits are used for polyclonal antibody production by nordc. Rabbit antibodies are very stable and can be stored for several days at room temperature. nordc adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
