Rabbit anti Mouse IgG1 IgG2a IgG2b IgG3 (Fc specific) antibody conjugated with Biotin
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Catalog numberRAM/IgG(Fc)/Bio
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Price:
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Size1 mL
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CategorySecondary Antibodies
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Long descriptionThe reactivity of the antiserum is directed to the Fc subunit of the IgG molecule. The antiserum contains antibodies to subclass-specific determinants as well as to determinants shared by two or more subclasses of IgG. In immunoelectrophoresis and double radial immunodiffusion, using various antiserum concentrations against normal mouse plasma, serum, and immunoglobulin fractions, no reaction is obtained with IgA, IgM and IgG/Fab fragments or any other serum protein. In immunocytochemical and immunohistochemical staining of IgG at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of mouse origin known to be of the IgG isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgG in mouse serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:500; in ELISA and comparable non-precipitating antibody-binding assays between 1:1,000 and 1:8,000.
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Antibody come fromPurified normal IgG, including all known subclasses, isolated from pooled mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
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Other descriptionBiotin-coupled purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. IgG protein concentration 10 mg/ml. Biotin/IgG protein molar ratio (B/P) is approximately 5.6. No foreign proteins added.
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ClonePolyclonal
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Antigen antibody binding interactionRabbit anti Mouse IgG1 IgG2a IgG2b IgG3 (Fc specific) antibody conjugated with Biotin Antibody
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Antibody is raised inRabbit
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Antibody s reacts withMouse
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Antibody s reacts with these speciesInter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has been tested in double radial immunodiffusion anti several sera with the following results: Bovine - Cat - Chicken - Dog - Duck - Goat - Guinea Pig + Hamster + Horse – Human - Monkey - pigeon - Rat + Sheep - Swine - Turkey - The absence of a reaction in double radial immunodiffusion does not exclude some reaction in more sensitive techniques.
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Antibody s specificityBiotin-conjugated IgG fraction of polyclonal Rabbit antiSerum to Mouse IgG, Fc specific
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Research interestVeterinary
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ApplicationELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting
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Antibody s suited forELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting.
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StorageThe lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate. Lyophilized at +4°C--at least 10 years. Reconstituted at or below -20°C--3-5 years. Reconstituted at +4°C--7 days
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Relevant referencesno information yet
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Protein numbersee ncbi
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WarningsThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. This is against a protein of mus musculus directed antibody.
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PropertiesIf you buy Antibodies supplied by nordc they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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ConjugationBiotinylated
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus, Oryctolagus cuniculus
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AboutRabbits are used for polyclonal antibody production by nordc. Rabbit antibodies are very stable and can be stored for several days at room temperature. nordc adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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French translationanticorps
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Gene target
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Short nameRabbit anti Mouse IgG1 IgG2a IgG2b IgG3 (Fc specific) antibody conjugated with
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Techniqueanti mouse, Antibody, Rabbit, Mouse, anti, FC, antibody to, anti mouse antibodies, antibodies against human proteins, antibodies for, antibody Conjugates, mouses
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HostRabbit, Rabbits
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Isotypenot specified, IgG1, IgG2a, IgG2b, IgG3
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LabelBiotin
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SpeciesMouse, Mouses
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Alternative nameproduction species: rabbit antibody to Mouse IgG1 IgG2a IgG2b IgG3 (fragment c specific) (antibody to-) coupled including biotinilated
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Alternative techniqueantibodies, rabbit-anti, murine
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MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data