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Category
Primary Antibodies
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Long description
Phosphatidic acid (PA), lysophosphatidic acid, ceramide 1-phosphate (C1P), and sphingosine 1-phosphate (S1P) are lipid mediators generated by phospholipases, sphingomyelinases, and lipid kinases. The major pathway for degradation of these lipids is dephosphorylation catalyzed by members of two classes (types 1 and 2) of phosphohydrolase activities (PAPs). cDNAs encoding two type 2 PAPs, PAP-2a and -2b, have been expressed by transient transfection and shown to catalyze hydrolysis of PA, C1P, and S1P.
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Antibody come from
Recombinant enyzme produced in E. coli
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Other description
Provided in 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, 10 mM glutathione. Antigen Immunoaffiinity Purification
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Clone
Polyclonal
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Antigen antibody binding interaction
Rabbit anti Lipid Phosphate Phosphohydrolase 2 Antibody
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Antibody is raised in
Rabbit
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Antibody s reacts with
Human, Porcine
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Application
Western Blot, Enzyme Immunoassay
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Antibody s suited for
4 nmole/min/µg of enzyme; Detemined using pNPP; Reaction conditions: 50 µM pNPP, 10 min incubation at 30¼C, 0.3 µg enzyme.
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Storage
-80ºC
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Relevant references
1: Yuki T, Ishihara S, Rumi M, Ortega-Cava Cesar F, Kadowaki Y, Kazumori H, Yuki M, Wada T, Miyaoka Y, Yoshino N, Kinoshita Y. Expression of midkine and receptor-like protein tyrosine phosphatase (RPTP)-beta genes in the rat stomach and the influence of rebamipide._x000B_Aliment Pharmacol Ther. 2003 Jul;18 Suppl 1:106-12. _x000B__x000B_2: Robles Y, Vivas-Mejia PE, Ortiz-Zuazaga HG, Felix J, Ramos X, Pena de Ortiz S._x000B_Hippocampal gene expression profiling in spatial discrimination learning. Neurobiol Learn Mem. 2003 Jul;80(1):80-95. _x000B__x000B_3: Hirayama T. Protein tyrosine phosphatase beta, a receptor for Helicobacter pylori vacA toxin._x000B_Keio J Med. 2002 Dec;51 Suppl 2:20-3. Review. _x000B__x000B_4: Shintani T, Maeda N, Noda M. Receptor-like protein tyrosine phosphatase gamma (RPTPgamma), but not PTPzeta/RPTPbeta, inhibits nerve-growth-factor-induced neurite outgrowth in PC12D cells._x000B_Dev Neurosci. 2001;23(1):55-69. _x000B__x000B_5: Chilton JK, Stoker AW. Expression of receptor protein tyrosine phosphatases in embryonic chick spinal cord. Mol Cell Neurosci. 2000 Oct;16(4):470-80. _x000B__x000B_6: Barnea G., Silvennoinen O., Shaanan B., Honegger A.M., Canoll P.D., D'Eustachio P., Morse B., Levy J.B., Laforgia S., Huebner K., Musacchio J.M., Sap J., Schlessinger J.;_x000B_ Identification of a carbonic anhydrase-like domain in the extracellular region of RPTP gamma defines a new subfamily of receptor tyrosine phosphatases.; Mol. Cell. Biol. 13:1497-1506(1993)._x000B__x000B_7: Kastury K., Ohta M., Lasota J., Moir D., Dorman T., Laforgia S., Druck T., Huebner K.;_x000B_ Structure of the human receptor tyrosine phosphatase gamma gene (PTPRG) and relation to the familial RCC t(3;8) chromosome translocation.; Genomics 32:225-235(1996)._x000B__x000B_8: Krueger N.X., Streuli M., Saito H.; Structural diversity and evolution of human receptor-like protein tyrosine phosphatases.;_x000B__x000B_9: Kaplan R., Morse B., Huebner K., Croce C., Howk R., Ravera M., Ricca G., Jaye M., Schlessinger J.; Cloning of three human tyrosine phosphatases reveals a multigene family of receptor-linked protein-tyrosine-phosphatases expressed in brain.;_x000B_Proc. Natl. Acad. Sci. U.S.A. 87:7000-7004(1990).
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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About
Rabbits are used for polyclonal antibody production by nordc. Rabbit antibodies are very stable and can be stored for several days at room temperature. nordc adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
