Rabbit anti Human Factor VIII related antigen (VWD)
#
-
Catalog numberRAHu/FVIII VWD
-
Price:
-
Size1 mL
-
-
CategoryPrimary Antibodies
-
Long descriptionThe defined antibody reactivity is restricted to VWF. In immunoelectrophoresis, bi-dimensional electrophoresis and radial immunodiffusion (Ouchterlony) against normal plasma, a single precipitin line is obtained which shows a reaction of identity with precipitated purified FVIII/VWF. No precipitation is obtained with plasma of type 1 congenital Von Willebrand’s disease. It is strongly recommended to perform the EID-assay with the special agarose (Agarose Nordic nr.4) Nordic High Resolution Buffer and High resolution-buffered Agarose, performance tested to assure reliable and reproducible results. The threshold of detection in this technique is about 0.15 mg per 100 ml. Haemophilia A and Von Willebrand’s disease (VWD) are known to be FVIII/VWF deficiencies. Immunologic determination of VWF antigen enables to discriminate between these two bleeding conditions. Haemophilia A patients lack FVIIIC but have a normal level of VWFag. In VWD, both FVIIIC and VWFag are reduced. Active and inactive FVIII/VWF, its breakdown products and inactivated FVIII/VWF-inhibitor complexes all express antigen determinants of VWFag and may be recognized by polyclonal antisera. Determination f plasma VWFag levels in addition to the level of FVIIIC can contribute also to the detection of the carrier state in haemophilia A. While the level of VWFag is normal or even elevated, the average concentration of FVIIIC of carriers is about half of normal. Various types and subtypes of congenital VWD have been described. Cases of acquired VWD have been reported in association with several clinical diseases including autoimmune disease, systemic lupus erythematosus, benign monoclonal gammapathy and Waldenström’s macroglobulinemia.
-
Antibody come fromHuman FVIII procoagulant activity (FVIIIC) is carried by a polypeptide non-covalently bound to a large carrier molecule (unit molecular weight 250 kD) known as the Von Willebrand factor (VWF). FVIII/VWF exists in plasma as a series of polymers with molecular weights > 1.100 kD. FVIIIC is probably formed in the liver, is unstable on storage but sufficiently stable in fresh frozen plasma as cryoprecipitate or FVIII concentrate. VWF is synthesized in megakaryocytes and vascular endothelial cells; it is also present in the alpha-granules and membranes of platelets, binding to specific sites on the activated platelet after its release. It is responsible for platelet adhesion to the vascular subendothelium. FVIIIC, but not VWF is completely consumed during coagulation ad is absent from serum. Polyclonal antisera are raised against FVIII/VWF purified from plasma. Freund’s complete adjuvant is used in the first step of the immunization procedure.
-
Other descriptionDelipidated, heat inactivated, lyophilized, stable whole serum. No preservative added. IgG protein concentration in the antiserum is 10 mg/ml. No foreign proteins added.
-
ClonePolyclonal
-
Antigen antibody binding interactionRabbit anti Human Factor VIII related antigen (VWD) Antibody
-
Antibody is raised inRabbit
-
Antibody s reacts withHuman
-
Antibody s reacts with these speciesThe antiSerum does not cross-react with any other Human plasma proteins as tested in gel-diffusion techniques. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.
-
Antibody s specificityPrecipitating polyclonal Rabbit antiSerum to Human Von Willebrand Factor (FVIII VWF).
-
Research interestCardiovascular, Immunology
-
ApplicationImmunoprecipitation
-
Antibody s suited forImmunoprecipitation. The antiserum concentration required in the gel is normally between 1 and 2%. In immunologic determinations of FVIII/VWF, plasma samples and all assay components must contain EDTA to stabilize the proteins The antiserum is standardized for use in the electroimmunodiffusion (EID, Laurell) test procedure for the quantitative determination of FVIII/VWF as described in the Nordic Recommended Working Procedure.
-
StorageThe lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day. Lyophilized at +4°C--at least 10 years. Reconstituted at or below -20°C--3-5 years. Reconstituted at +4°C--7 days.
-
Relevant referencesno information yet
-
Protein numberP04275
-
WarningsThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
-
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk. Antigens are peptides or recombinant or native dependent on the production method. Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.
-
PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
-
AboutRabbits are used for polyclonal antibody production by nordc. Rabbit antibodies are very stable and can be stored for several days at room temperature. nordc adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
-
Latin nameOryctolagus cuniculus
-
Gene target
-
Gene symbolCOX8A, G3BP1, MARCHF8
-
Short nameRabbit anti Factor VIII antigen (VWD)
-
Techniqueanti human, antigen, Rabbit, anti, antibody to, anti human antibodies, antigenes
-
HostRabbit, Rabbits
-
Isotypenot specified
-
Labelunlabeled
-
SpeciesHuman, Humans
-
Alternative nameproduction species: rabbit antibody to H. sapiens Factor VIII related protein (VWD)
-
Alternative techniqueantigenes, rabbit-anti, antibodies
-
Gene info
-
Identity
-
Gene
-
Long gene namecytochrome c oxidase subunit 8A
-
Synonyms gene
-
Synonyms gene name
- cytochrome c oxidase subunit VIII
- cytochrome c oxidase subunit VIIIA (ubiquitous)
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year1989-06-30
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Mitochondrial complex IV: cytochrome c oxidase subunits
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameG3BP stress granule assembly factor 1
-
Synonyms gene name
- GTPase activating protein (SH3 domain) binding protein 1
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year2006-10-25
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- RNA binding motif containing
- Endoribonucleases
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene namemembrane associated ring-CH-type finger 8
-
Synonyms gene
-
Synonyms gene name
- c-mir, cellular modulator of immune recognition
- membrane-associated ring finger (C3HC4) 8
- membrane-associated ring finger (C3HC4) 8, E3 ubiquitin protein ligase
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2003-11-12
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Ring finger proteins
- Membrane associated ring-CH-type fingers
-
VEGA ID
MeSH Data
-
Name
-
ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data