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Category
Primary Antibodies
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Long description
Voltage-dependent calcium channels (VDCCs) are large (>400 kDa) heteromers which contain, minimally, three core subunits 1, 2/ , in a 1:1:1 stoichiometry1. Expression of VDCC gene products in Xenopus oocytes, or transfected cells shows that the 1 subunits contain the ion channel pore while the auxiliary 2/ and subunits confer optimal cell surface expression and channel kinetics1. Until recently, the only exception to the above paradigm was the skeletal muscle VDCC, which, in addition to the 1, 2/ , core motif, also has an additional tightly associated integral membrane glycoprotein subunit termed 1. Upon co-expression with the 1.1, 2/ 1, 1a subunits of the skeletal muscle VDCC, subunits alter the peak currents, and the kinetics of channel activation and inactivation with the overall effect being a normalisation of currents to those resembling the endogenous channel2. Together, these results suggest that subunits modulate skeletal muscle VDCCs by stabilising their conformation. _x000B_The 2 subunits, or stargazin, the product of a gene mutated in the stargazer mouse, is a homolog of the 1 channel and is selectively expressed in the brain. It is considered to be a putative neuronal Ca2+ channel subtype mainly based on its homology to the 1.
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Antibody come from
n/a
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Other description
Provided as a sterile solution in 50 mM HEPES containing 2 mM EDTA, 100 mM NaCl and 1 mM MgCl2 (pH 7.5). Ammonium Sulfate Precipitation
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Clone
Polyclonal
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Antigen antibody binding interaction
Rabbit anti g2 Calcium Channel Antibody
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Antibody is raised in
Rabbit
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Antibody s reacts with
Human
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Application
Western Blot
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Antibody s suited for
Membrane preparation for use in [35S]GTP S binding assays using lysophosphatidic acid as the ligand for the receptor. Prepared from lysed RH7777 cells and contains purified membrane protein with G-protein coupled EDG4 receptors. NOTE: The protocol below has been condensed. Please see Ref. 3 for more detailed information.
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Storage
-20ºC
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Relevant references
1. Goetzl EJ and An, S. Diversity of cellular receptors and functions for the lysophospholipid growth factos lysophosphatidic acid and sphingosine 1-phosphate. FASEB J. 1998, 12, 1589-98._x000B__x000B_2. An, S., Goetzl, E.J., Lee, H. Signaling mechanisms and molecular characteristics of G protein-coupled receptors for lysophosphatidic acid and sphingosine 1-phosphate. J. Cell Biochem. Suppl. 1998, 30-31, 147-57._x000B__x000B_3. Parrill, A.L., et al. 'Identification of Edg1 receptor residues that recognize sphingosine 1-phosphate.' J. Biol. Chem. 2000, 275, 39379-39384.
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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About
Rabbits are used for polyclonal antibody production by nordc. Rabbit antibodies are very stable and can be stored for several days at room temperature. nordc adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
