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Category
Primary Antibodies
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Long description
Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD)- containing adapter molecules and members of the ICE/CED-3 protease family. Caspases-8 (FLICE) and -10 (FLICE2) are two pivotal members in the ICE/CED-3 protease family. FLICE-inhibitory proteins were identified in virus and human and designated v-FLIPs and FLIP, respectively1,2. The human FLIP was also cloned by several labs independently and termed Casper, I-FLICE, FLAME-1, CASH and CLARP3-7. FLIP contains two death effector domains (DEDs) and a caspase-like domain. FLIP interacts with adapter protein FADD and caspase-8 and Ð10, and potently inhibits apoptosis induced by all known death receptors. Four splice variants of c-FLIPs have been identified and termed FLIP , , , and , respectively8.
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Antibody come from
Synthetic peptide corresponding to amino acids 447 to 464 of the C-terminal of the human FLIP /FLIPL form.
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Other description
Provided in phosphate buffered saline solution containing 0.02% sodium azide as a preservative. Antigen Immunoaffiinity Purification
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Clone
Polyclonal
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Antigen antibody binding interaction
Rabbit anti flip (CT) (I-flICE) (flICE Inhibitory Protein) Antibody
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Antibody is raised in
Rabbit
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Antibody s reacts with
Human, Mouse, Rat
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Application
Western Blot
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Antibody s suited for
Detects FLIP by Western blot at 0.25 to 1 µg/ml. Detects a 55 kDa band in HeLa whole cell lysates. Antibody recognizes the FLIP only. Optimal concentration should be evaluated by serial dilutions.
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Storage
-20ºC
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Relevant references
1. Thome, M., et al. 'Viral FLICE-inhibitory proteins (FLIPs) prevent apoptosis induced by death receptors.' Nature 1997, 386, 517-521_x000B__x000B_2. Irmler, M., et al. 'Inhibition of death receptor signals by cellular FLIP.' Nature 1997, 388, 190-195 _x000B__x000B_3. Shu, H.B., et al. 'Casper is a FADD- and caspase-related inducer of apoptosis.' Immunity 1997, 6, 751-763 _x000B__x000B_4. Hu, S., et al. 'I-FLICE, a novel inhibitor of tumor necrosis factor receptor-1- and CD-95-induced apoptosis.' J. Biol. Chem. 1997, 272, 17255-17257 _x000B__x000B_5. Srinivasula, S.M., et al.. 'FLAME-1, a novel FADD-like anti-apoptotic molecule that regulates Fas/TNFR1-induced apoptosis.' J. Biol. Chem. 1997, 272, 18542-18545 _x000B__x000B_6. Goltsev, Y.V., et al. 'CASH, a novel caspase homologue with death effector domains.' J. Biol. Chem. 1997, 272, 19641-19644 _x000B__x000B_7. Inohara, N., et al. 'CLARP, a death effector domain-containing protein interacts with caspase-8 and regulates apoptosis.' Proc. Natl. Acad. Sci. USA 1997, 94, 10717-10722 _x000B__x000B_8. Wallach, D. 'Apoptosis. Placing death under control.' Nature 1997, 388, 123
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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About
Rabbits are used for polyclonal antibody production by nordc. Rabbit antibodies are very stable and can be stored for several days at room temperature. nordc adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
