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Category
Primary Antibodies
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Long description
ERK (extracellular signal regulated kinase), also known as MAPK (mitogen-activated protein kinase) has two closely related isoforms of 44 kDa and 42 kDa, respectively. These kinases belong to a family of serine/threonine kinases that are activated upon treatment of cells with a large variety of stimuli including mitogens, hormones, growth factors, cytokines, and bioactive peptides. Cell stimulation induces the activation of a signaling cascade, the downstream effects of which have been linked to the regulation of cell growth and differentiation as well as the cytoskeleton. ERK1 and ERK2 are phosphorylated within the activation loop on both a Threonine and a Tyrosine residue (within a Thr-Glu-Tyr motif) by MEKs (MAPK/ERK kinases), thereby greatly elevating the activity of ERK1&2.
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Antibody come from
Recombinant rat interleukin-2.
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Other description
Purified IgG in phosphate buffered saline, pH 7.4. Purified from rabbit serum by peptide affinity chromatography.
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Clone
Polyclonal
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Antigen antibody binding interaction
Rabbit anti ERK1/2 (pan) Antibody
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Antibody is raised in
Rabbit
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Antibody s reacts with
Human, Mouse, Rat and Bovine ERK1&2. Other species have not been tested.
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Application
Western Blot
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Antibody s suited for
Antibody can be used for ELISA, Western blot and Dot bot. For dot blot, a 1:10,000 dilution of the antibody will detect 5 ng of protein. Antibody is also suitable for neutralization studies. Optimal concentration should be evaluated by serial dilutions. Further dilutions should be made in medium or buffered solution with carrier protein such as PBS with 0.1-1.0% BSA.
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Storage
-80ºC
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Relevant references
Tzianabos, A.O., P.R. Russell, A.B. Onderdonk, F. Gibson, C. Cywes, M. Chan,_x000B_R.W. Finberg, and D.L. Kasper (1999) IL-2 mediates protection against abscess_x000B_formation in an experimental model of sepsis. J. Immunol. 163:893-897.
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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About
Rabbits are used for polyclonal antibody production by nordc. Rabbit antibodies are very stable and can be stored for several days at room temperature. nordc adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
