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Category
Primary Antibodies
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Long description
DNA Topoisomerase 1 releases the supercoiling and torsional tension of DNA introduced during DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. It introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells.
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Antibody come from
Synthetic peptide derived from human fibulin-3 protein
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Other description
Provided as solution in phosphate buffered saline with 0.08% sodium azide. Antigen Immunoaffinity Purification
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Clone
Polyclonal
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Antigen antibody binding interaction
Rabbit anti DNA Topoisomerase 1 Antibody
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Antibody is raised in
Rabbit
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Antibody s reacts with
Human
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Application
Immunohistochemistry (paraffin-embedded)
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Antibody s suited for
Optimal concentration should be evaluated by serial dilutions.
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Storage
-20ºC
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Relevant references
1. Ikegawa, S., et al. Structure and chromosomal assignment of the human S1-5 gene (FBNL) that is highly homologous to fibrillin. Genomics 35: 590-592 (1996)_x000B__x000B_2. Stone, E.M., et al. A single EFEMP1 mutation associated with both malattia leventinese and Doyne honeycomb retinal dystrophy. Nat. Genet. 22: 199-202 (1999)_x000B__x000B_3. Giltay, R., et al. Sequence, recombinant expression and tissue localization of two novel extracellular matrix proteins, Fibulin-3 and Fibulin-4. Matrix Biol. 18: 469-480 (1999)_x000B__x000B_4. Matsumoto, M., et al. Dominant radial drusen and Arg345Trp EFEMP1 mutation. Am. J. Ophthalmol. 131: 810-812 (2001)_x000B__x000B_5. Marmorstein, L.Y., et al. Aberrant accumulation of EFEMP1 underlies drusen formation in malattia leventinese and age-related macular degeneration. Proc. Natl. Acad. Sci. USA 99: 13067-13072 (2002)_x000B__x000B_6. Marmorstein, L.Y., et al. Association of EFEMP1 with malattia leventinese and age-related macular degeneration: a mini-review. Ophthalmic Genet. 25: 219-226 (2004)_x000B__x000B_7. Klenotic, P.A., et al. Tissue inhibitor of metalloproteinases-3 (TIMP-3) is a binding partner of epithelial growth factor-containing fibulin-like extracellular matrix protein 1 EFEMP1). Implications for macular degenerations. J. Biol. Chem. 279: 30469-30473 (2004)_x000B__x000B_8. Narendran, N., et al. Analysis of the EFEMP1 gene in individuals and families with early onset drusen. Eye 19: 11-15 (2005)_x000B_
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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About
Rabbits are used for polyclonal antibody production by nordc. Rabbit antibodies are very stable and can be stored for several days at room temperature. nordc adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
