Rabbit anti cyclin dependent kinase 4

• Catalog number
  X1216P
• Price
  Please ask
• Size
  100 μg

• Category
  Primary Antibodies
• Long description
  p34cdk4 is a major catalytic subunit of mammalian D-type cyclins which is expressed by a gene found in human chromosome 12q13. The cdk4 protein is constitutively expressed throughout the cell cycle and it is the assembly and accumulation of functional cdk4/cyclin D complexes which is rate limiting for the progression through the G1 phase of the cell cycle. Once the complex is formed it must be activated by the phosphorylation of threonine 172 on the cdk4 subunit, a reaction mediated by a cdk-activating kinase (CAK). Complex formation and phosphorylation are independently regulated events. The phosphorylation of the cdk4 subunit is subject to regulation of both stimulatory and inhibitory cytokine signals, thereby allowing cdk4 to function as a mid G1 checkpoint. Independent of phosphorylation, cdk4 is also regulated by the binding of p16Ink4/mts1 while in complex with cyclin D.
• Antibody come from
  TrpE-fusion protein containing the C-terminal 164 amino acids of human Cdk4
• Other description
  Provided as solution in phosphate buffered saline with 0.08% sodium azide. Ammonium Sulfate Precipitation
• Clone
  Polyclonal
• Antigen antibody binding interaction
  Rabbit anti cyclin dependent kinase 4 Antibody
• Antibody is raised in
  Rabbit
• Antibody s reacts with
  Human
• Antibody s reacts with these species
  This antibody doesn't cross react with other species
• Antibody s specificity
  No Data Available
• Application
  Western Blot, Immunoprecipitation
• Antibody s suited for
  Detects Cdk4 by Western blot in HeLa cell lysates. Can also be used for immunoprecipitation. Optimal concentration should be evaluated by serial dilutions.
• Storage
  -20ºC
• Relevant references
  1. Ando, K. and Griffin, J.D. Cdk4 integrates growth stimulatory and inhibitory signals during G1 phase of hematopoietic cells. Oncogene 1995, 10, 751-755._x000B__x000B_2. Tam, S.W., et al. Differential expression and cell cycle regulation of the cyclin-dependent kinase 4 inhibitor p16Ink4. Cancer Res. 1994, 54, 5816-5820_x000B__x000B_3. Khatib, Z.A., et al. Coamplification of the CDK4 gene with MDM2 and GLI in human sarcomas. Cancer Res. 1993, 53, 5535-5541
• Protein number
  see ncbi
• Warnings
  This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
• Antibody s link
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
• About
  Rabbits are used for polyclonal antibody production by nordc. Rabbit antibodies are very stable and can be stored for several days at room temperature. nordc adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
• Latin name
  Oryctolagus cuniculus

• Gene target
  cyclin   dependent   kinase  
• Gene symbol
  CDKL4, CDK4P1, CDK4
• Short name
  Rabbit anti cyclin dependent kinase 4
• Technique
  Rabbit, anti, antibody to
• Host
  Rabbit, Rabbits
• Isotype
  not specified
• Label
  unlabeled
• Alternative name
  production species: rabbit antibody to cyclin dependent phosphorylation catalyst 4
• Alternative technique
  rabbit-anti, antibodies

Gene info

• Identity
  HGNC:19287
• Gene
  CDKL4
• Long gene name
  cyclin dependent kinase like 4
• Synonyms gene name
  • cyclin-dependent kinase-like 4
• Locus
  2p22.1
• Discovery year
  2004-05-24
• Entrez gene record
  344387
• RefSeq identity
  • NM_001346911
• Classification
  • Cyclin dependent kinases
• VEGA ID
  OTTHUMG00000133574

Gene info

• Identity
  HGNC:19040
• Gene
  CDK4P1
• Long gene name
  cyclin dependent kinase 4 pseudogene 1
• Synonyms gene
  • CDK4PS
• Synonyms gene name
  • cyclin dependent kinase 4 pseudogene
• Locus
  1p21.1
• Discovery year
  2003-07-21
• Entrez gene record
  359941
• VEGA ID
  OTTHUMG00000011002

Gene info

• Identity
  HGNC:1773
• Gene
  CDK4
• Long gene name
  cyclin dependent kinase 4
• Synonyms
  • PSK-J3
• GenBank acession
  • M14505
• Locus
  12q14.1
• Discovery year
  1993-07-28
• Entrez gene record
  1019
• Pubmed identfication
  • 8275715
• RefSeq identity
  • NM_000075
• Classification
  • Cyclin dependent kinases
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000170382
• Locus Specific Databases
  • LRG_490

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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