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Category
Primary Antibodies
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Long description
Autotaxin (ATX), a tumor cell motility- stimulating protein is an ecto/exo-enzyme with significant homology to the plasma cell membrane differentiation antigen PC-1. ATX is a 99kDa core protein and may run as a 125-kDa glycoprotein when isolated from the human melanoma cell line (A2058). ATX, like PC-1 can hydrolyze the type I phosphodiesterase substrate p-nitrophenyl thymidine-5'-monophosphate. Autotaxin has a novel motility- regulating function for this class of ecto/exo-enzymes.
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Antibody come from
Synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho-Thr202 and Tyr204 of ERK/MAPK.
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Other description
Provided in HEPES (pH 7.5) solution containing 150 mM NaCl, 100 µg per ml BSA and 50% glycerol . Ammonium Sulfate Precipitation
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Clone
Polyclonal
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Antigen antibody binding interaction
Rabbit anti Autotaxin Antibody
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Antibody is raised in
Rabbit
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Antibody s reacts with
Human
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Application
Western Blot, Enzyme Immunoassay
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Antibody s suited for
Antibody should be used at a 1:1000 dilution to provide for 10 miniblots in Western blotting and dot blots. Antibody detects only phosphorylated protein and does not detect non-phosphorylated protein as shown by the lack of ability of a non-phospho peptide to block the antibody activity. Optimal concentration may be evaluated by serial dilutions.
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Storage
-20ºC
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Relevant references
1. Ahn, N.G. 'The MAP kinase cascade. Discovery of a new signal transduction pathway.' Mol. Cell Biochem. 1993, 127, 201-209_x000B__x000B_2. Adams, J.P. and Sweatt, J.D. 'Molecular psychology: Roles for the ERK MAP kinase cascade in memory.' Annu. Rev. Pharmacol. Toxicol. 2002, 42, 135-163_x000B__x000B_3. Atkins, C.M., et al. 'The MAPK cascade is required for mammalian associative learning.' Nat. Neurosci. 1998, 1, 602-609_x000B__x000B_4. Charest, D.L., et al. 'Molecular cloning, expression, and characterization of the human mitogen-activated protein kinase p44erk1.' Mol. Cell Biol. 1993, 13, 4679-4690_x000B__x000B_5. Johnson, G.L. and Lapadat, R. 'Mitogen-activated protein kinase pathways mediated by ERK, JNK, and p38 protein kinases.' Science 2002, 298, 1911-1912_x000B__x000B_6. Tanogue, T.J. and Nishida, E. 'Molecular recognitions in the MAP kinase cascades.' Cellular Signaling 2003, 15, 455-462_x000B__x000B_7. Ahn, NG (1993) The MAP kinase cascade. Discovery of a new signal transduction pathway. Mol Cell Biochem 127- 128:201-209.
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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About
Rabbits are used for polyclonal antibody production by nordc. Rabbit antibodies are very stable and can be stored for several days at room temperature. nordc adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
