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Category
Primary Antibodies
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Long description
Recognizes a protein of 85kDa, identified as the X-Ray Repair Cross Complementing (XRCC1). The C-terminal 96 amino acids of XRCC1 are necessary and sufficient for the specific interaction with DNA ligase III of 103kDa. The region of interaction with DNA polymerase beta is located within residues 84-183 in the N-terminal half of the XRCC1 protein. Reportedly, Poly (ADP-ribose) polymerase also interacts with XRCC1. In testis XRCC1 is expressed at high levels. The XRCC1 gene plays a role for the sensitivity of mammalian cell lines toward ionizing irradiation. Cells with a mutation of this gene present with decreased single strand break repair and reduced recombination repair, they show increased double strand breaks, and sister chromatid exchange is increased up to 10-fold. The XRCC1 might serve as a scaffold protein during base excision-repair.
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Antibody come from
Synthetic peptide derived from the rat Kv1.3 potassium channel conjugated to KLH
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Other description
Provided as solution in phosphate buffered saline with 0.08% sodium azide. Protein A/G Chromatography
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Clone
144
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Antigen antibody binding interaction
Mouse anti XRCC1 (X-Ray Repair Cross Complementing 1 Protein) Antibody
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Antibody is raised in
Mouse
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Antibody s reacts with
Human
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Application
Western Blot, Immunohistochemistry (Paraffin Sections)
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Antibody s suited for
This antibody can be used for Western blotting (5-10 µg/ml). Optimal concentration should be evaluated by serial dilutions.
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Storage
-20ºC
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Relevant references
1. Stuhmer, W et.al. 'Molecular basis of functional diversity of voltage-gated potassium_x000B_channels in mammalian brain.' EMBO J. 8 (11), 3235-3244 (1989)_x000B__x000B_2. Swanson, R et.al. 'Cloning and expression of cDNA and genomic clones encoding threedelayed rectifier potassium channels in rat brain.' Neuron 4 (6), 929-939 (1990)_x000B__x000B_3. Douglass, J et.al. 'Characterization and functional expression of a rat genomic DNA_x000B_clone encoding a lymphocyte potassium channel' J. Immunol. 144 (12), 4841-4850 (1990)_x000B__x000B_4. Chang, M.C. et.al 'Regulation of Kv1.3 channels in activated human T lymphocytes by Ca(2+)-dependent pathways.' Cell Physiol Biochem.11(3):123-34. 2001_x000B__x000B_5. Butchason,I., et. al. 'Angular methoxy-substituted furo- and pyranoquinolinones as blockers of the voltage-gated potassium channel Kv1.3.' J. Med. Chem. 44(8):1249-56 (2001)._x000B_
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
