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Category
Primary Antibodies
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Long description
The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, fibronectin, laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. MMP-9 (also designated 92-kDa type IV collagenase or gelatinase B) has been shown to degrade bone collagens in concert with MMP-1 (also designated interstitial collagenase, fibroblast collagenase or collagenase-1), and cysteine proteases and may play a role in bone osteoclastic resorption. MMP-1 is down-regulated by p53, and abnormality of p53 expression may contribute to joint degradation in rheumatoid arthritis by regulating MMP-1 expression.
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Antibody come from
Full length human protein with C-terminal FLAG tag.
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Other description
Provided in 40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 40 µg/ml Flag peptide, 3 mM DTT and 20% glycerol. Protein A/G Chromatography
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Clone
2C3
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Antigen antibody binding interaction
Mouse anti Matrix Metalloproteinase 9 (MMP9) Antibody
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Antibody is raised in
Mouse
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Antibody s reacts with
Human
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Application
Western Blot, Immunohistochemistry (Paraffin sections)
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Antibody s suited for
142 pmole/min/µg.
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Storage
-20ºC
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Relevant references
1. Lamour NF, Chalfant CE. ÔCeramide-1-phosphate: The missing link in eicosanoid biosynthesis and inflammation.Õ Mol Interv. 2005 Dec;5(6):358-67. _x000B__x000B_2.Kim JW, Inagaki Y, Mitsutake S, Maezawa N, Katsumura S, Ryu YW, Park CS, Taniguchi M, Igarashi Y. ÔSuppression of mast cell degranulation by a novel ceramide kinase inhibitor, the F-12509A olefin isomer K1.Õ Biochim Biophys Acta. 2005 Dec 30;1738(1-3):82-90. Epub 2005 Nov 14. _x000B__x000B_3. Van Overloop H, Gijsbers S, Van Veldhoven PP. ÔFurther characterization of mammalian ceramide kinase: substrate delivery and (stereo)specificity, tissue distribution, and subcellular localization studies.Õ_x000B_J Lipid Res. 2006 Feb;47(2):268-83. Epub 2005 Nov 3. _x000B__x000B_4. Chalfant CE, Spiegel S. ÔSphingosine 1-phosphate and ceramide 1-phosphate: expanding roles in cell signaling.Õ J Cell Sci. 2005 Oct 15;118(Pt 20):4605-12. Review. _x000B__x000B_5. Mitsutake S, Igarashi Y. ÔCalmodulin is involved in the Ca2+-dependent activation of ceramide kinase as a calcium sensor.Õ J Biol Chem. 2005 Dec 9;280(49):40436-41. Epub 2005 Oct 3. _x000B__x000B_6. Wijesinghe DS, Massiello A, Subramanian P, Szulc Z, Bielawska A, Chalfant CE. ÔSubstrate specificity of human ceramide kinase.Õ_x000B_J Lipid Res. 2005 Dec;46(12):2706-16. Epub 2005 Sep 18. _x000B__x000B_7.Kim TJ, Mitsutake S, Kato M, Igarashi Y. ÔThe leucine 10 residue in the pleckstrin homology domain of ceramide kinase is crucial for its catalytic activity.Õ FEBS Lett. 2005 Aug 15;579(20):4383-8.
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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Gene target
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Gene symbol
MMP9
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Short name
Mouse anti Matrix Metalloproteinase 9 (MMP9)
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Technique
Mouse, anti, antibody to, mouses
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Host
mouse
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Isotype
IgG1
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Label
unlabeled
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Species
Mouse, Mouses
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Alternative name
Mouse antibody to Matrix Metalloproteinase 9 (matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase))
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Alternative technique
murine, antibodies
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Alternative to gene target
matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase), CLG4B and GELB and MANDP2 and MMP-9, MMP9 and IDBG-78722 and ENSG00000100985 and 4318, identical protein binding, Extracellular, Mmp9 and IDBG-212451 and ENSMUSG00000017737 and 17395, MMP9 and IDBG-642990 and ENSBTAG00000020676 and 282871
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